| 无偿献血人群隐匿性乙型肝炎病毒感染调查 |
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| 引用本文： | 臧伟伟,齐文革,杨贺,张玉敏. 无偿献血人群隐匿性乙型肝炎病毒感染调查[J]. 实用肝脏病杂志, 2022, 25(4): 496-499. DOI: 10.3969/j.issn.1672-5069.2022.04.011 |
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| 作者姓名： | 臧伟伟 齐文革 杨贺 张玉敏 |
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| 作者单位： | 071000 河北省保定市中心血站质量管理科(臧伟伟,齐文革);河北大学附属医院检验科(杨贺);唐山市人民医院检验科(张玉敏) |
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| 基金项目： | *河北省卫生健康委员会科研基金资助项目(编号:20201538) |
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| 摘 要： | 目的 调查无偿献血人群隐匿性乙型肝炎病毒感染(OBI)情况。方法 2021年1月～2021年12月我站无偿献血者血液样本107397份，采用两种ELISA法检测试剂盒进行HBsAg的初次筛查，采用核酸检测(NAT)法对两次HBsAg结果均为阴性的样本进行HBV DNA检测。对血清HBsAg阴性而HBV DNA阳性样本进行HBV血清学标志物检测，并采用实时荧光定量聚合酶链式反应(PCR)法检测核酸和病毒基因分型。结果 在筛查的107397例无偿献血人群血样本中，经血清标志物检测后确认为OBI者29例(0.27‰);血清抗-HBc阳性者12例(35.3%),抗-HBe/抗-HBc阳性者8例(23.5%),抗-HBs/抗-HBc阳性者6例(17.7%),抗-HBs/抗-HBe/抗-HBc阳性者3例(8.8%);19～29岁年龄段献血人群OBI感染率为0.09‰,30～39岁人群为0.32‰,40～49岁人群为0.39‰,50～55岁年龄段献血人群OBI感染率为0.41‰,且该年龄段重复献血者OBI感染率为0.31‰;血清抗-HBs/抗-HBc阳性和抗-HBs/抗-HBe/抗-HBc OBI献...
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| 关 键 词： | 隐匿性乙型肝炎病毒感染 乙型肝炎病毒表面抗原 酶联免疫吸附法 核酸检测 无偿献血人群 |
| 收稿时间： | 2022-04-14 |
Prevalence of occult hepatitis B viral infections in blood donors |
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| Zang Weiwei,Qi Wenge,Yang He,et al. Prevalence of occult hepatitis B viral infections in blood donors[J]. Journal of Clinical Hepatology, 2022, 25(4): 496-499. DOI: 10.3969/j.issn.1672-5069.2022.04.011 |
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| Authors: | Zang Weiwei Qi Wenge Yang He et al |
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| Affiliation: | Department of Quality Management, Central Blood Station, Baoding 071000, Hebei Province, China |
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| Abstract: | Objective The aim of this study was to investigate the prevalence of occult hepatitis B viral infections (OBI) in blood donors (BD). Methods 107397 blood samples were collected in our Blood Station between January and December 2021. The initial serum screening of HBsAg was conducted by two ELISA kits, and serum HBV DNA loads was assayed by NAT. For serum HBV DNA positive samples, serum HBV markers were repeatedly detected and HBV genotype was determined by real-time fluorescence quantitative polymerase chain reaction (PCR). Results Out of the 107397 blood samples, the OBI was found in 29 cases (0.27‰) as defined by serum HBV DNA positive; the re-testing of the positive blood showed that serum anti-HBc positive in 12 cases (35.3%), anti-HBe/anti-HBc positive in 8 cases (23.5%), anti-HBs/anti-HBc positive in 6 cases (17.7%) and anti-HBs/anti-HBe/anti-HBc positive in 3 cases (8.8%); the prevalence of OBI in individuals at 19-29 year old was 0.09‰, in those at 30-39 was 0.32‰, in at 40-49 was 0.39‰ and in at 50-55 was 0.41‰, and in persons at age of 50-55 with more than one time blood donor, the prevalence of OBI was 0.31‰; the percentages of serum HBV DNA loads greater than 1000 IU/mL in OBI individuals with serum anti-HBs/anti-HBc positive and with serum anti-HBs/anti-HBe/anti-HBc positive were 33.3% and 66.7%, significantly higher than 0.0% in those with serum anti-HBc positive (P<0.05) or with serum anti-HBe/anti-HBc positive (0.0%, P<0.05); out of the 29 OBI persons, the genotype C infection accounted for 62.1% and genotype B infection for 27.6%. Conclusion The prevalence of OBI in blood donors is a challenge for public health security, and the cost-efficacy of all-scale detection of serum HBV DNA loads in this setting needs further investigation. The quality and sensitivity of conventional ELISA kits should be improved. |
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| Keywords: | Occult hepatitis B virus infection Hepatitis B surface antigen Enzyme-linked immunosorbent assay Nucleic acid test Blood donors |
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