| lncRNA SNHG17 靶向 miR-525-5p 对人绒毛膜滋养层细胞增殖、迁移和侵袭的影响 |
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| 引用本文： | 叶玉锦,黄丽珊,黄巧如,吴明秀. lncRNA SNHG17 靶向 miR-525-5p 对人绒毛膜滋养层细胞增殖、迁移和侵袭的影响[J]. 医学分子生物学杂志, 2022, 19(6): 452-456. DOI: 10.3870/j.issn.1672-8009.2022.06.003 |
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| 作者姓名： | 叶玉锦 黄丽珊 黄巧如 吴明秀 |
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| 作者单位： | 东莞市人民医院妇产科 广东省东莞市, 523000 |
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| 基金项目： | 广东省医学科学技术研究基金 (No. C2019097) |
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| 摘 要： | 目的 探讨 lncRNA SNHG17 对人绒毛膜滋养层细胞生长及转移的影响及其可能作用机制。 方法 qRT-PCR 检测正常胎盘组织、 子痫前期胎盘组织中 lncRNA SNHG17 和 miR-525-5p 表达。 体外培养人绒毛膜滋养层细胞 HTR-8 / SVneo, 分别转染 si-SNHG17、 anti-miR-525-5p 及其阴性对照; 采用平板克隆形成实验、 划痕实验与 Transwell 实验分别检测细胞增殖、 迁移及侵袭能力; 双荧光素酶报告实验检测 miR-525-5p与 SNHG17 的靶向关系。 结果 与正常胎盘组织比较, 子痫前期胎盘组织中 lncRNA SNHG17 表达升高,miR-525-5p 表达降低 (P< 0. 05)。 转染 si-SNHG17 后细胞克隆形成数和侵袭细胞数增多, 划痕愈合率升高(P< 0. 05)。 lncRNA SNHG17 靶向调控 miR-525-5p; 共转染 si-SNHG17 和 anti-miR-525-5p 后细胞克隆形成数和侵袭细胞数减少, 划痕愈合率降低 (P< 0. 05)。 结论 沉默 lncRNA SNHG17 可通过促进 miR-525-5p 表达而促进人绒毛膜滋养层细胞增殖、 迁移及侵袭。
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| 关 键 词： | 子痫前期 人绒毛膜滋养层细胞 lncRNA SNHG17 miR-525-5p 细胞增殖 迁移 侵袭 |
Effect of lncRNA SNHG17 on Proliferation,Migration and Invasionof Human Chorionic Trophoblast Cells by Targeting miR-525-5p |
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| YE Yujin,HUANG Lishan,HUANG Qiaoru,WU Mingxiu. Effect of lncRNA SNHG17 on Proliferation,Migration and Invasionof Human Chorionic Trophoblast Cells by Targeting miR-525-5p[J]. Journal of Medical Molecular Biology, 2022, 19(6): 452-456. DOI: 10.3870/j.issn.1672-8009.2022.06.003 |
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| Authors: | YE Yujin HUANG Lishan HUANG Qiaoru WU Mingxiu |
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| Affiliation: | Department of Obstetrics and Gynecology, Dongguan People’s Hospital, Dongguan, Guangdong,523000, China |
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| Abstract: | Objective To investigate the effect of lncRNA SNHG17 on the growth and metastasis of human chorionic trophoblast cells and its possible mechanism. Methods The expression levels of lncRNA SNHG17 and miR-525-5p in normal placental tissues and preeclampsia placental tissues were detected by qRT-PCR. Human chorionic trophoblast cells HTR-8 / SVneo were culturedinvitroand were transfected with the si-SNHG17, anti-miR-525-5p and their negative controls, respectively. The plate colony formation assay, scratch assay and transwell assay were used to detectthe cell proliferation, migration and invasion, respectively. Dual-luciferase reporter assay was usedto verify the targeting relationship between miR-525-5p and SNHG17. Results Compared with thenormal placenta tissues, the expression level of lncRNA SNHG17 in preeclampsia placenta tissueswas increased, while the expression level of miR-525-5p was decreased (P< 0. 05). After transfection with si-SNHG17, the number of colonies and invasive cells were increased, the wound healingrate was increased (P< 0. 05). lncRNA SNHG17 could bind with miR-525-5p. After the co-transfection of si-SNHG17 and anti-miR-525-5p, the number of colonies and invasive cells were decreased, the wound healing rate was decreased ( P < 0. 05 ). Conclusion Silencing lncRNASNHG17 could promote the proliferation, migration and invasion of human chorionic trophoblastcells by upregulation of miR-525-5p. |
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| Keywords: | preeclampsia human chorionic trophoblast cells lncRNA SNHG17 miR-525-5p cell proliferation migration invasion |
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