| 双氢青蒿素诱导人急性髓系白血病HL-60细胞凋亡 |
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| 引用本文： | 陈伟;胡亮杉;王玲;杜苑苑;曹东林. 双氢青蒿素诱导人急性髓系白血病HL-60细胞凋亡[J]. 广东医学, 2011, 32(13) |
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| 作者姓名： | 陈伟 胡亮杉 王玲 杜苑苑 曹东林 |
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| 作者单位： | 广东省第二人民医院
南方医科大学珠江医院血液科 |
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| 摘 要： | 目的：探讨双氢青蒿素诱导人急性髓系白血病HL-60细胞的凋亡作用和机制。方法：用二甲氧唑黄（XTT）细胞增殖及细胞毒性检测试剂盒检测双氢青蒿素对HL-60细胞生长的抑制作用，AnnexinV-FITC/PI染色流式细胞仪检测细胞凋亡，流式细胞仪检测细胞内线粒体膜电位变化，分光光度法检测细胞Caspase3活性变化。结果：双氢青蒿素对HL-60细胞生长有抑制作用，呈剂量依赖性，并能诱导HL-60细胞凋亡，使线粒体膜去极化，增强Caspase3活性。结论：双氢青蒿素能抑制人急性髓系白血病HL-60细胞生长，并诱导细胞凋亡，可能和线粒体凋亡通路有关。
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| 关 键 词： | 双氢青蒿素 人急性髓系白血病 线粒体途径 凋亡 |
Effects of dihydroartemisinin on apoptosis of human acute myeloblast leukemia HL-60 cells in vitro |
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| Abstract: | Objective: To explore the effects and mechanism of dihydroartemisinin on apoptosis of human acute myeloblastic leukemia HL-60 cells. Methods: The growth inhibition of HL-60 cells treated with dihydroartemisinin was detected by XTT cell proliferation and cytotoxicity assay kit; The apoptosis of HL-60 cells induced by curcumin was detected by flow cytometry through the staining of AnnexinV-FITC/PI; The change of mitochondria membrane potential was analysed by flow cytometry through the staining of JC-1; The activity of Caspase3 was detected by ELISA. Results: Dihydroartemisinin could inhibit HL-60 cells proliferation with a concentration-dependent manner and could induce the apoptosis of HL-60 cells with depolarizing mitochondria membrane potential and acitviting Caspase3. Conclusion: Dihydroartemisinin could inhibit HL-60 cells proliferation and induce HL-60 cells apoptosis perhaps through mitochondria apoptosis pathway. |
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