| 硕大利什曼原虫无鞭毛体蛋白的基因克隆与序列分析 |
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| 引用本文： | 成军,斯崇文,王勤环,刘妍,钟彦伟,杨继珍. 硕大利什曼原虫无鞭毛体蛋白的基因克隆与序列分析[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(1): 30-32 |
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| 作者姓名： | 成军 斯崇文 王勤环 刘妍 钟彦伟 杨继珍 |
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| 作者单位： | 1. 解放军302医院传染病研究所基因治疗研究中心,北京,100039
2. 北京医科大学第一医院感染疾病科,北京,100034 |
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| 摘 要： | [目的 ]克隆硕大利什曼原虫无鞭毛体蛋白 (amastin)的编码基因序列。 [方法 ]应用核苷酸序列数据库 (GenBank)和表达序列末端片段数据库 (dbEST)的计算机检索与DNA文库的杂交筛选方法。 [结果 ]从dbEST数据库中获得一段 30 9nt的来源于硕大利什曼原虫的基因片段 ,据此设计探针 ,筛选硕大利什曼原虫的DNA文库 ,获得硕大利什曼原虫无鞭毛体蛋白的编码基因。其开放读码框架由 5 5 2个核苷酸组成 ,编码产物由183个氨基酸残基组成。序列分析表明 ,硕大利什曼原虫与锥虫无鞭毛体蛋白一级结构的同源性为 2 3 5 %。 [结论 ]克隆的基因系硕大利什曼原虫表面蛋白编码基因 ,即无鞭毛体蛋白的编码基因。
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| 关 键 词： | 利什曼原虫 无鞭毛体 基因文库 基因克隆 |
| 文章编号： | 1000-7423(2000)-01-0030-03 |
| 修稿时间： | 1998-04-02 |
CLONING AND SEQUENCE ANALYSIS OF A GENE ENCODING AMASTIN FROM LEISHMANIA MAJOR |
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| CHEN Jun,SI Chong-wen,WANG Qin-huan,LIU Yan,ZHONG Yan-wei,YANG Ji-zhen. CLONING AND SEQUENCE ANALYSIS OF A GENE ENCODING AMASTIN FROM LEISHMANIA MAJOR[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2000, 18(1): 30-32 |
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| Authors: | CHEN Jun SI Chong-wen WANG Qin-huan LIU Yan ZHONG Yan-wei YANG Ji-zhen |
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| Affiliation: | CHEN Jun(Gene Therapy Research Center,The Institute of Infectious Diseases,302 Hospital of PLA,Beijing 100039)SI Chong-wen,WANG Qin-huan(Department of Infectious Diaeases.The First School of Clinical Medicine, Beijing Medical University,Beijing 100034)LIU Yan,ZHONG Yan-wei,YANG Ji-zhen(Gene Therapy Research Center,The Institute of Infectious Diseases,302 Hospital of PLA,Beijing 100039) |
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| Abstract: | Objective]To clone a gene encoding surface protein from Leishmania major .[Methods] Using T cruzi amastin DNA sequence as a reference,computer search was done on GenBank and dbEST databases by using BLAST path. A Leishmania major DNA library has been constructed and screened by in situ colony hybridization.[Results] A 309nt DNA fragment from Leishmania major was found in dbEST. Leishmania major DNA library was screened using specific primers synthesized according to 309 nt DNA sequence, and a full length coding sequence for Leishmania major amastin was cloned. The coding sequence consisted of 552 nt, and translated into 183 amino acid residues. The homology is 23 5% at amino acid sequence level between Leishmania major and T cruzi amastins. [Conclusion] A full length amastin coding gene for Leishmania major has been cloned. |
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| Keywords: | Leishmania amastin GenBank database gene cloning. |
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