| 双向排斥反应联体动物模型的建立 |
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| 引用本文： | 熊兵,陈华德,赖文,熊仕秋,郑少逸,高辉,卞徽宁,刘族安. 双向排斥反应联体动物模型的建立[J]. 南方医科大学学报, 2005, 25(12): 1511-1513 |
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| 作者姓名： | 熊兵 陈华德 赖文 熊仕秋 郑少逸 高辉 卞徽宁 刘族安 |
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| 作者单位： | 1. 广东省人民医院烧伤科, 广东, 广州, 510080;2. 中山大学基础医学院免疫教研室, 广东, 广州, 510080 |
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| 基金项目： | 广东省医药卫生科研项目 |
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| 摘 要： | 目的 探讨在异基因成年小鼠之间建立联体模型用以研究双向排斥反应。方法 54只雄性C57BL/6(H-2b,C57)小鼠和54只雌性Balb/c(H-2d)小鼠配对分为3组:1组(注射生理盐水)、2组(注射脾细胞+环磷酰胺)、3组(注射脾细胞+环磷酰胺+环孢素A)。于术中经尾静脉注入对方的脾细胞;术前3d和术后1周每日分别腹腔注射环孢素A(30mg·kg·d-1);术后2d腹腔注射环磷酰胺(150mg/kg)1次。对经以上处理的部分联体小鼠在术后1周后分开,相互植皮。观察联体维持时间,并分别检测其混合淋巴细胞反应和诱导迟发型超敏反应(DTH)。结果 第3组联体时间明显延长;第3组联体小鼠DTH,2、3组显示明显抑制,但3组小鼠抑制程度明显比第2组小鼠深。结论 应用免疫抑制剂,可成功建立双向排斥反应联体动物模型。
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| 关 键 词： | 免疫抑制 动物模型 排斥反应 |
| 文章编号： | 1000-2588(2005)12-1511-03 |
| 修稿时间： | 2005-08-13 |
Establishing a parabiosis animal model of two-way paradigm |
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| XIONG Bing,CHEN Hua-de,LAI Wen,XIONG Shi-qiu,ZHEN Shao-yi,GAO Hui,BIAN Hui-ning,LIU Zu-an. Establishing a parabiosis animal model of two-way paradigm[J]. Journal of Southern Medical University, 2005, 25(12): 1511-1513 |
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| Authors: | XIONG Bing CHEN Hua-de LAI Wen XIONG Shi-qiu ZHEN Shao-yi GAO Hui BIAN Hui-ning LIU Zu-an |
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| Abstract: | Objective To establish a parabiosis model between allogenic conspecific adult mice to study two-way paradigm. Methods Fifty-four female Balb/c mice and 54 male C57BL/6 mice were paired and equally divided into 3 groups, namely group 1 with normal saline (NS) injection, group 2 with injections of spleen cells and cyclophosphamide (CP), and group 3 injected with spleen cells, CP, and cyclosporin A (CsA). The treatments were performed by injecting the spleen cells from one of the mice in a pair into the other via tail vein and vise versa, and two days after the operation, CP (150 mg/kg) was injected intraperitoneally. Intraperitoneal CsA (30 mg/kg daily) injection was given starting from 2 days before till 7 days after the operation. Twelve of the 18 pairs of parabiosis mice in each group were separated after 1 week, and part of the skin were transplanted to each other. The maintenance of parabiosis was observed in the other 6 pairs of parabiosis mice were observed. Mixed lymphocyte reaction (MLR) and delayed-type hypersensitivity (DTH) were observed and studied in the separated mice. Result The duration of parabiosis maintenance and skin survival of the group 3 was significantly longer than those in the other two groups, and group 3 showed suppressed MLR and DTH. Conclusion With the application of immunosuppressants, we have successfully established the two-way paradigm model in mice. |
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| Keywords: | parabiosis chimerism adult tolerance |
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