Molecular Detection and Identification of Intimin Alleles in Pathogenic Escherichia coli by Multiplex PCR |
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| Authors: | Sean D. Reid David J. Betting Thomas S. Whittam |
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| Affiliation: | Institute of Molecular Evolutionary Genetics, Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA. |
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| Abstract: | A multiplex PCR was designed to detect the eae gene and simultaneously identify specific alleles in pathogenic Escherichia coli. The method was tested on 87 strains representing the diarrheagenic E. coli clones. The results show that the PCR assay accurately detects eae and resolves alleles encoding the alpha, beta, and gamma intimin variants. |
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