| 幼虫冰冻切片抗原间接荧光抗体试验诊断旋毛虫病 |
| |
| 引用本文： | 崔晶,王中全,武峰,晋雪香,张鹏远,杨瑞琴,刘俊. 幼虫冰冻切片抗原间接荧光抗体试验诊断旋毛虫病[J]. 中国寄生虫学与寄生虫病杂志, 1999, 17(1): 28-31 |
| |
| 作者姓名： | 崔晶 王中全 武峰 晋雪香 张鹏远 杨瑞琴 刘俊 |
| |
| 作者单位： | 1 河南医科大学寄生虫学教研室 郑州450052;2 河南医科大学第二附属医院 郑州450003;3 河南医科大学第一附属医院 郑州450052 |
| |
| 摘 要： | 目的：评价幼虫冰冻切片抗原间接荧光抗体试验（ＩＦＡＴ）诊断旋毛虫病的价值。方法：应用纯净幼虫冰冻切片间接荧光抗体试验检测感染旋毛虫小鼠和旋毛虫病患者血清中的抗旋毛虫抗体,在患者发病后及应用阿苯达唑治疗后不同时间采集血清,以其他寄生虫病患者和健康人血清为对照。结果：１５只小鼠感染旋毛虫后２ｗｋ即可检出抗体,４６７例旋毛虫病患者的抗体阳性率为８６．５％,而丝虫病、包虫病患者各４５例和健康人血清５６份均为阴性。４８份并殖吸虫病患者的交叉反应率为１６．６７％。旋毛虫病患者发病后１ｗｋ的抗体阳性率为７０．２１％,至发病后２ｗｋ、３ｗｋ及４ｗｋ则分别升至９１．０％、９５．８％及１００％。幼虫冰冻切片的抗原活性于－２０℃可保存至５年。旋毛虫病患者治疗后１ｗｋ抗体阳性率从治疗前的８７．５％上升到１００％,血清抗体滴度比治疗前也有明显升高,治疗后１个月的抗体转阴率为２４％,至治疗后４个月达７５％。结论：幼虫冰冻切片间接荧光抗体试验诊断旋毛虫病具有较高的敏感性和特异性,并可用于疗效考核
|
| 关 键 词： | 旋毛虫病 血清学诊断 间接荧光抗体试验 |
DIAGNOSIS OF TRICHINOSIS BY INDIRECT FLUORESCENT ANTIBODY TEST WITH TRICHINELLA LARVA SECTION * |
| |
| CUI Jing,WANG Zhongquan,WU Feng,JIN Xuexiang,ZHANG Pengyuan,YANG Ruiqin,LIU Jun. DIAGNOSIS OF TRICHINOSIS BY INDIRECT FLUORESCENT ANTIBODY TEST WITH TRICHINELLA LARVA SECTION *[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1999, 17(1): 28-31 |
| |
| Authors: | CUI Jing WANG Zhongquan WU Feng JIN Xuexiang ZHANG Pengyuan YANG Ruiqin LIU Jun |
| |
| Affiliation: | Department of Parasitology, Henan Medical University, Zhengzhou 450052. |
| |
| Abstract: | AIM: To evaluate the value of indirect fluorescent antibody test (IFAT) in diagnosing trichinosis. METHODS: IFAT using frozen section of the purified Trichinella larvae as antigen was employed to detect anti-Trichinella antibodies in sera from mice infected with Trichinella spiralis and the patients with trichinosis. Sera from patients with trichinosis were collected at different time intervals after onset of the disease and after treatment with albendazole. Sera from patients with other parasitic diseases and healthy individuals were used as control. RESULTS: The anti-Trichinella antibodies were detected in 15 infected mice as early as 2 weeks after infection, and in 86.5% of 467 patients with trichinosis. Sera from patients with filariasis, echinococcosis and healthy controls were all negative. 8 of 48 (16.7%) cases with paragonimiasis showed positive cross reaction. The antibody positive rate of patients with trichinosis was 70.2% one week after onset of disease and increased to 91.0%, 95.8% and 100% at two, three and four weeks after onset, respectively. The frozen section of the larvae can be stored at -20 degrees C for 5 years without loss of antigenicity. The IFAT positive rate was increased from 87.5% before treatment to 100% one week after treatment, and the serum antibody titers were elevated as well. The antibody negative conversion rate of the patients was 24% at one month after teratment and increased to 75% at four months after treatment. CONCLUSION: IFAT using frozen section of the purified Trichinella larvae as antigen is highly sensitive and specific for the diagnosis of trichinosis and evaluation of the therapeutic effect. |
| |
| Keywords: | Trichinosis')" >Trichinosis serodiagnosis ')" >IFAT |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》浏览原始摘要信息 |
|
点击此处可从《中国寄生虫学与寄生虫病杂志》下载免费的PDF全文 |
|
