Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci |
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| Authors: | Kariyama R Mitsuhata R Chow J W Clewell D B Kumon H |
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| Affiliation: | Section of Infection Control, Okayama University Hospital, Okayama 700-8558, Japan. kariyama@med.okayama-u.ac.jp |
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| Abstract: | Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci. |
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