| 顺铂诱导肾小管上皮细胞毒性及Bcl-2对抗细胞凋亡作用机理研究 |
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| 引用本文： | 冯乐平,乔伟,DONG Zheng. 顺铂诱导肾小管上皮细胞毒性及Bcl-2对抗细胞凋亡作用机理研究[J]. 基础医学与临床, 2008, 28(2): 167-171 |
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| 作者姓名： | 冯乐平 乔伟 DONG Zheng |
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| 作者单位： | 1. 桂林医学院,生物技术学院,广西桂林,541004
2. 佐治亚医学院,美国,30912 |
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| 基金项目： | 致谢：感谢美国Medical College of Geogia细胞生物学系的Zheng Dong教授提供的帮助和指导 |
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| 摘 要： | 目的探讨顺铂诱导肾小管上皮细胞(RPTC)凋亡的作用机制和BCL-2基因抑制顺铂诱导细胞凋亡的作用途径。方法应用不同亚细胞结构分布的BCL-2基因体外转染RPTC,激光共焦显微技术和免疫荧光技术分析BCL-2(X)基因表达产物在RPTC线粒体和内质网上的分布定位,采用Hoechst33258染色并进行细胞凋亡计数。结果不同畸变的BCL-2(BCL-acta,BCL-cb5)分别定位于RPTC的线粒体、内质网,BCL-nt同时定位于上述两种细胞器。BCL-cb5组可见更多BAX被激活,同时可见较多碎裂细胞核。定位于线粒体上的BCL-acta明显抑制顺铂对RPTC诱导的细胞凋亡(P<0.05)。结论BCL-2抑制顺铂诱导RPTC凋亡主要是通过线粒体途径起作用。
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| 关 键 词： | 细胞凋亡 顺铂 BCL-2基因 细胞信号传导 |
| 文章编号： | 1001-6325（2008）02-0167-05 |
| 收稿时间： | 2007-04-23 |
| 修稿时间： | 2007-08-10 |
RPTC cells toxicity induced by cisplatin and anti-apoptosis mechanism of BCL-2 |
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| FENG Le-ping,QIAO Wei,DONG Zheng. RPTC cells toxicity induced by cisplatin and anti-apoptosis mechanism of BCL-2[J]. Basic Medical Sciences and Clinics, 2008, 28(2): 167-171 |
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| Authors: | FENG Le-ping QIAO Wei DONG Zheng |
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| Abstract: | Objective To explore the mechnism of toxicity induced by cisplatin on renal proximal tubular cells(RPTC) line and anti-apoptosis mechanism of BCL-2 with transfection of different mutant BCL-2 in vitro.MethodsRPTC was translocated with different mutants BCL-2(s).Cell apoptosis induced by cisplatin on RPTC was analyzed with confocal and flurencent microscope.The cell apoptosis was measured with Hoechst33258 after treatment with cisplatin.Results Different mutant BCL-2(BCL-acta,BCL-cb5)were translocated on mitochondrial and Endoplasmic Reticulum(ER) respectively.BCL-acta protected RPTC from apoptosis induced by cisplatin more easily than BCL-cb5 group in a time-dependant manner(P<0.05).Conclusion Cisplatin can induce RPTC apoptosis obivously through a mitochondrial signaling payhway and BCL-2 can protect more RPTC from apoptosis in the mitochondrial manner other than in ER manner |
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| Keywords: | cell apoptosis cisplatin BCL-2 gene signaling transduction |
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