| 日本血吸虫童虫cDNA文库的免疫筛选及其生物信息学分析 |
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| 引用本文： | 张云霞,王晓楠,张薇娜,沈际佳. 日本血吸虫童虫cDNA文库的免疫筛选及其生物信息学分析[J]. 中国血吸虫病防治杂志, 2008, 20(6): 423-426 |
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| 作者姓名： | 张云霞 王晓楠 张薇娜 沈际佳 |
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| 作者单位： | 安徽医科大学基础医学院病原生物学教研室（合肥230032） |
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| 基金项目： | 国家自然科学基金，安徽省科技攻关项目 |
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| 摘 要： | 目的筛选新的可能具有诊断意义的血吸虫蛋白分子,并对其进行生物信息学分析。方法以血吸虫病人血清筛选日本血吸虫15d肝期童虫cDNA文库,对阳性克隆插入片段的核苷酸进行序列分析,将结果通过Internet送入NCBI GenBank进行同源性比较并利用蛋白质分析软件进行生物信息学分析。结果经3轮筛选,共获15个阳性克隆,对其进行测序,获得11个基因,其中5个为编码日本血吸虫线粒体的基因,1个为编码日本血吸虫肌球蛋白的基因,其他5个分别为编码SjCHGC05315、SjCHGC01371、SjCHGC04782、SjCHGC05166、SjCHGC09769的基因。结论从日本血吸虫肝期童虫cDNA文库中筛选到一批日本血吸虫基因,为血吸虫病新的诊断候选分子的研究提供了材料。
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| 关 键 词： | 日本血吸虫 童虫 cDNA文库 免疫筛选 生物信息学 |
Immunoscreening of schistosomulum cDNA library of Schistosoma japonicum and preliminary identificaton |
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| Zhang Yun-xia,Wang Xiao-nan,Zhang Wei-na,Shen Ji-jia. Immunoscreening of schistosomulum cDNA library of Schistosoma japonicum and preliminary identificaton[J]. Chinese journal of schistosomiasis control, 2008, 20(6): 423-426 |
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| Authors: | Zhang Yun-xia Wang Xiao-nan Zhang Wei-na Shen Ji-jia |
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| Affiliation: | (Department of Pathogenic Biology ,Anhui Medical University, Hefei 230032, China) |
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| Abstract: | Objective To obtain genes encoding the novel molecules for diagnosis of schistosomiasis. Methods The cDNA library of Schistosoma japonicum （Sj） sehistosomula of day 15 post-infection was screened with positive patients sera,and the inserts of positive clones were sequenced. Then the sequences were compared with all sequences in GenBank database by Internet. The positive clones were analyzed by the software in bioinformatics. Results After three rounds of screening, 15 positive clones were obtained, and among them 11 clones had the meaningful sequences, in which 5 genes coding for Sj mitochondrion, 1 gene coding for Sj myosin and the others were SjCHGC05315,SjCHGC01371, SjCHGC04782, SjCHGC05166, SjCHGC09769, respectively. Conclusion Immunological screening by using positive patients sera can be used to discover Sj antigen genes with a potential value for diagnosis. |
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| Keywords: | Schistosoma japonicum Schistosomulum cDNA library Immunoscreening Bioinformatics |
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