| nm23-H1基因对人肺腺癌A549细胞增殖和侵袭的抑制作用 |
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| 引用本文： | 刘秋英,吴志聪,胡红梅,熊盛,张美英,袁茵,刘美莉,王一飞. nm23-H1基因对人肺腺癌A549细胞增殖和侵袭的抑制作用[J]. 中国病理生理杂志, 2006, 22(7): 1335-1339. DOI: 1000-4718 |
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| 作者姓名： | 刘秋英 吴志聪 胡红梅 熊盛 张美英 袁茵 刘美莉 王一飞 |
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| 作者单位： | 1. 暨南大学,生物医药研究开发基地,广东,广州,510632
2. 暨南大学,生命科学院化学系,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金;广东省博士启动基金;广东省广州市科技攻关项目;中国科学院资助项目 |
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| 摘 要： | 目的:研究转染nm23-H1基因对体外培养A549细胞增殖和侵袭的抑制作用及其机理。方法:构建nm23-H1基因的真核表达载体,转染到体外培养的人肺腺癌细胞A549中,通过G418筛选出稳定表达克隆,RT-PCR及免疫组化检测nm23-H1在细胞内的表达情况。绘制细胞生长曲线检测nm23-H1基因对细胞生长的影响,流式细胞仪检测细胞周期,原子力显微镜观察细胞膜表面伪足的超微结构。结果:转染后的肿瘤细胞能稳定表达nm23-H1基因,抑制了肿瘤细胞的增殖。nm23-H1基因没有诱导细胞凋亡但使G1期细胞增加而S期细胞减少,停滞于G0期。转染nm23-H1基因后细胞边缘的伪足减少。结论:nm23-H1基因能抑制体外培养的A549肿瘤细胞的增殖,可能通过改变细胞表面结构减弱细胞的侵袭能力。
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| 关 键 词： | 基因 nm23-H1 肿瘤侵袭 肺肿瘤 A549细胞 |
| 文章编号： | 1000-4718(2006)07-1335-05 |
| 收稿时间： | 2004-09-29 |
| 修稿时间： | 2004-09-29 |
Inhibitory effects of nm23-H1 gene on proliferation and invasion of A549 cell line |
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| LIU Qiu-ying,WU Zhi-cong,HU Hong-mei,XIONG Sheng,ZHANG Mei-ying,YUAN Yin,LIU Mei-li,WANG Yi-fei. Inhibitory effects of nm23-H1 gene on proliferation and invasion of A549 cell line[J]. Chinese Journal of Pathophysiology, 2006, 22(7): 1335-1339. DOI: 1000-4718 |
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| Authors: | LIU Qiu-ying WU Zhi-cong HU Hong-mei XIONG Sheng ZHANG Mei-ying YUAN Yin LIU Mei-li WANG Yi-fei |
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| Abstract: | AIM: To study the inhibitory effects of nm23-H1 gene on proliferation and invasion of human lung adenocarcinoma A549 cell line. METHODS: Recombinant eukaryotic expression vector pcDNA3.1-nm23-H1 containing full length of human nm23-H1 cDNA was constructed and transfected into a human lung adenocarcinoma A549 cell line by lipofectamine. Cell strain that expressed nm23-H1 stably was screened out by G418 and named pcDNA-nm23-A549. Expression of nm23-H1 was identified by RT-PCR and immunohistochemistry. Growth curves were drawn to detect the inhibitory effects on cell proliferation. Cell cycle of pcDNA-nm23-A549 was examined by flow cytometry. Atomic force microscopy was used to observe the filopodia on the surface of the cells. RESULTS: Introduction of nm23-H1 obviously inhibited the proliferation of A549. Expression of nm23-H1 did not induce apotosis in A549 cells but increased the percentage of phase G_1 cells and decreased phase S cells. Meanwhile, phase G_1 to phase S transition was restrained. Filopodia in the cell surface was much fewer and its structure changed in cells transformed. CONCLUSION: nm23-H1 is capable of inhibiting A549 proliferation and decreasing its metastatic ability, probably by interfering with cell cycle and cell surface structure. |
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| Keywords: | Genes nm23-H1 Neoplasm invasiveness Lung neoplasms A549 cells |
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