| 扁塑藤素抑制人胶质母细胞瘤U251细胞增殖的机制探讨 |
| |
| 引用本文： | 徐高峰,鲍 钢,白晓斌,祁 磊,谢万福,李瑞春. 扁塑藤素抑制人胶质母细胞瘤U251细胞增殖的机制探讨[J]. 现代肿瘤医学, 2019, 0(3): 363-366. DOI: 10.3969/j.issn.1672-4992.2019.03.001 |
| |
| 作者姓名： | 徐高峰 鲍 钢 白晓斌 祁 磊 谢万福 李瑞春 |
| |
| 作者单位： | 西安交通大学医学院第一附属医院神经外科，陕西 西安 710061 |
| |
| 基金项目： | 陕西省科技研究发展（攻关）计划项目（编号:2014K11-01-01-03） |
| |
| 摘 要： | 目的:探讨扁塑藤素对人胶质母细胞瘤U251细胞增殖的影响及其机制。方法:2、4和8μmol/L的扁塑藤素作用U251细胞24 h后,使用四甲基偶氮唑蓝(MTT)法检测U251细胞活力; Cell cycle staining Kit检测U251细胞周期;免疫印迹法检测增殖细胞核抗原(PCNA)蛋白和p-PI3K、PI3K、p-AKT以及AKT蛋白表达。结果:2、4和8μmol/L的扁塑藤素显著降低U251细胞的活力,且具有浓度依赖性。扁塑藤素可下调U251细胞PCNA蛋白的表达。扁塑藤素处理U251细胞后,G_1期细胞数明显增加,S期细胞数明显减少。扁塑藤素处理可明显降低U251细胞p-PI3K/PI3K、p-AKT/AKT的比值。结论:扁塑藤素可能通过抑制PI3K/AKT信号通路的活化抑制人胶质母细胞瘤的细胞增殖。
|
| 关 键 词： | 扁塑藤素 胶质母细胞瘤 细胞增殖 PI3K/AKT信号通路 |
The mechanism of pristimerin inhibits proliferation of human glioblastoma U251 cells |
| |
| Xu Gaofeng,Bao Gang,Bai Xiaobin,Qi Lei,Xie Wanfu,Li Ruichun. The mechanism of pristimerin inhibits proliferation of human glioblastoma U251 cells[J]. Journal of Modern Oncology, 2019, 0(3): 363-366. DOI: 10.3969/j.issn.1672-4992.2019.03.001 |
| |
| Authors: | Xu Gaofeng Bao Gang Bai Xiaobin Qi Lei Xie Wanfu Li Ruichun |
| |
| Affiliation: | Department of Neurosurgery，the First Affiliated Hospital of Xi'an Jiaotong University，Shaanxi Xi'an 710061，China. |
| |
| Abstract: | Objective:To explore the impact of pristimerin on cell proliferation of U251 cells.Methods:After treating with different concentrations of pristimerin（2，4，8 μmol/L) for 24 h，the viability of U251 cells was measured using MTT assay.Cell cycle was detected by cell cycle staining Kit.The expression levels of PCNA（proliferating cell nuclear antigen)，p-PI3K，PI3K，p-AKT and AKT were determined by Western blot.Results:Compared with the control group，pristimerin markedly reduced the cell viability of U251 in a dose dependent way.Additionally，the expression levels of PCNA protein were obviously downregulated in pristimerin-treated U251 cells.We also found that the cell numbers in G1 period were obviously increased while the cell numbers in S period were markedly decreased in U251 cells treated by pristimerin.Eventually，the ratios of p-PI3K/PI3K and p-AKT/AKT were markedly reduced in pristimerin-treated U251 cells.Conclusion:Pristimerin can inhibit cell proliferation of human glioblastoma by inhibiting the activation of PI3K/AKT signalling. |
| |
| Keywords: | pristimerin human glioblastoma cell proliferation PI3K/AKT signalling |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《现代肿瘤医学》浏览原始摘要信息 |
|
点击此处可从《现代肿瘤医学》下载免费的PDF全文 |
