| 上调NRG1对甲状腺癌细胞增殖和凋亡的影响及可能机制 |
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| 引用本文： | 樊新龙1,郭 囡1,高 鑫1,杨 骁1,石 刚2,赵月皎1. 上调NRG1对甲状腺癌细胞增殖和凋亡的影响及可能机制[J]. 现代肿瘤医学, 2019, 0(1): 21-25. DOI: 10.3969/j.issn.1672-4992.2019.01.006 |
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| 作者姓名： | 樊新龙1 郭 囡1 高 鑫1 杨 骁1 石 刚2 赵月皎1 |
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| 作者单位： | 1.中国医科大学肿瘤医院，辽宁省肿瘤医院头颈外科；2.结直肠外科，辽宁 沈阳 110042 |
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| 基金项目： | 2017年辽宁省重点研发计划指导计划项目（编号:2017225056）；辽宁省博士科研启动基金（编号:201601417）；2017年沈阳市科技计划项目（编号:17-230-9-52）；2018年沈阳市科技计划项目（编号:18-014-4-75） |
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| 摘 要： | 目的:探讨神经调节蛋白1（neuregulin 1，NRG1）对甲状腺癌细胞系MDA-T32和B-CPAP增殖和凋亡的影响及可能机制。方法:构建pCDNA3.1-NRG1过表达载体，脂质体法转染甲状腺癌细胞系MDA-T32和B-CPAP，qRT-PCR检查转染组与对照组NRG1基因表达，Western blotting法检测各组细胞NRG1蛋白表达。用CCK-8法检测细胞的增殖能力。流式细胞术检测Annexin V／PI双染各组MDA-T32细胞的凋亡情况。Western blotting法检测凋亡相关蛋白Caspase-3、Bax和Bcl-2的表达。结果: pCDNA3.1-NRG1成功转染MDA-T32和B-CPAP细胞，转染后qRT-PCR和Western blotting结果显示NRG1基因和蛋白表达水平上调。高表达NRG1能抑制MDA-T32和B-CPAP细胞的增殖。流式细胞术结果显示，NRG1诱导MDA-T32和B-CPAP细胞凋亡。Western blotting结果显示NRG1高表达可以促进MDA-T32和B-CPAP细胞Caspase-3、Bax的表达，抑制Bcl-2的表达。结论: NRG1能够抑制甲状腺癌细胞的增殖，通过调节线粒体凋亡通路诱导凋亡。
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| 关 键 词： | NRG1 甲状腺癌 增殖 凋亡 |
The effect of upregulation of NRG1 on proliferation and apoptosis of thyroid cancer cells and its mechanism |
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| Fan Xinlong1,Guo Nan1,Gao Xin1,Yang Xiao1,Shi Gang2,Zhao Yuejiao1. The effect of upregulation of NRG1 on proliferation and apoptosis of thyroid cancer cells and its mechanism[J]. Journal of Modern Oncology, 2019, 0(1): 21-25. DOI: 10.3969/j.issn.1672-4992.2019.01.006 |
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| Authors: | Fan Xinlong1 Guo Nan1 Gao Xin1 Yang Xiao1 Shi Gang2 Zhao Yuejiao1 |
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| Affiliation: | 1.Department of Head and Neck Surgery；2.Department of Colorectal Surgery，Cancer Hospital of China Medical University，Liaoning Cancer Hospital & Institute，Liaoning Shenyang 110042，China. |
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| Abstract: | Objective:To investigate the effect and possible mechanism of neuregulin 1 (NGR1) on proliferation and apoptosis of thyroid cancer cell line MDA-T32 and B-CPAP.Methods:There was construction of the NRG1 overexpression vector pCDNA3.1-NRG1，transfection of thyroid cancer cell lines MDA-T32 and B-CPAP by Liposomal，then detection of the gene and protein expression of NRG1 by qRT-PCR and Western blotting.The MDA-T32 and B-CPAP cells proliferation were detected by CCK-8.The apoptosis of MDA-T32 and B-CPAP cells were detected by Flow cytometry with Annexin V/PI double staining.The expression of Caspase-3，Bax and Bcl-2 were detected by Western blotting.Results:The MDA-T32 and B-CPAP cells were successfully transfected by pCDNA3.1-NRG1.The results of qRT-PCR and Western blotting after transfection showed that the expression levels of NRG1 gene and protein were up-regulated.High expression of NRG1 could inhibit the proliferation of MDA-T32 and B-CPAP cells.Flow cytometry results showed that up-regulated NRG1 induced apoptosis of MDA-T32 and B-CPAP cells.Moreover，Western blotting results showed that high expression of NRG1 could promote the expression of Caspase-3 and Bax in MDA-T32 and B-CPAP cells，and inhibit the expression of Bcl-2.Conclusion:NRG1 could inhibit the proliferation of thyroid cancer cells and induce apoptosis through regulating the mitochondrial apoptosis pathway. |
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| Keywords: | NRG1 thyroid cancer proliferation apoptosis |
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