| miR-21的功能分析及其在结肠癌中的临床意义 |
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| 引用本文： | 韦皓棠1,王佳雷1,倪 敏1,郭尔娜2. miR-21的功能分析及其在结肠癌中的临床意义[J]. 现代肿瘤医学, 2019, 0(1): 104-107. DOI: 10.3969/j.issn.1672-4992.2019.01.026 |
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| 作者姓名： | 韦皓棠1 王佳雷1 倪 敏1 郭尔娜2 |
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| 作者单位： | 1.广西医科大学第三附属医院胃肠外科，广西 南宁 530031；2.广西医科大学国际教育学院，广西 南宁 530021 |
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| 基金项目： | 广西壮族自治区卫生计生委项目（编号:Z2015198）；南宁市科学技术局项目（编号:20173018-3） |
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| 摘 要： | 目的:分析miR-21的功能及其在结肠癌中的临床意义。方法:采用miRWalk在线工具预测和筛选miR-21的靶基因，并使用DAVID分析靶基因的功能和信号通路；检测结肠癌患者和健康对照人群血清以及癌组织和癌旁组织标本的miR-21的表达水平；使用TCGA数据库中结肠癌的数据，分析miR-21表达水平与临床病理参数的关系以及与患者预后的关系。结果:共筛选出429个miR-21的靶基因，靶基因功能分析提示这些基因主要参与了正性调节代谢过程、蛋白结合等。结肠癌患者血清miR-21表达水平显著高于健康对照人群，差异有统计学意义（P<0.05）。TCGA数据显示，结肠癌组织中的miR-21表达水平与肿瘤的临床分期、肿瘤侵袭程度、淋巴结转移分期、远处转移分期无显著差异（P>0.05）。生存分析显示组织中高表达miR-21的患者其生存时间较低表达者短（P<0.05）。结论:miR-21调控多个肿瘤发生相关的基因，在结肠癌发生过程中具有重要的调控作用。结肠癌患者miR-21表达水平显著高于健康人群，且miR-21高表达的患者预后差。
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| 关 键 词： | 结肠癌 miR-21 功能分析 预后分析 |
Function analysis of miR-21 and the clinical significance in colon cancer |
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| Wei Haotang1,Wang Jialei1,Ni Min1,Guo Erna2. Function analysis of miR-21 and the clinical significance in colon cancer[J]. Journal of Modern Oncology, 2019, 0(1): 104-107. DOI: 10.3969/j.issn.1672-4992.2019.01.026 |
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| Authors: | Wei Haotang1 Wang Jialei1 Ni Min1 Guo Erna2 |
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| Affiliation: | 1.Department of Gastrointestinal Surgery，the Third Affiliated Hospital of Guangxi Medical University，Guangxi Nanning 530031，China;2.International Education College of Guangxi Medical University，Guangxi Nanning 530021，China. |
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| Abstract: | Objective:To analyze the function of miR-21 and the clinical significance in colon cancer.Methods:The target genes of miR-21 were screened by the miRWalk online tool，and the gene function and signal pathways were analyzed by DAVID tool.The serum levels of miR-21 were tested and compared in colon cancer patients and healthy controls.The data of colon cancer was download from TCGA database，and the relation of miR-21 with clinicopathological parameters were analyzed，the relation of miR-21 and prognosis of colon cancer patients was also analyzed.Results:429 validated target genes of miR-21 were screened.These target genes were involving the positive regulation of metabolic process,protein binding.The serum levels of miR-21 were significantly increased in colon patients compared with that in healthy controls(P＜0.05).The data of TCGA revealed that the expression of miR-21 in colon cancer tissue has not significant difference in clinical stage，tumor invasive，lymph node metastasis，distant metastasis of colon cancer(P＞0.05).Survival analysis showed that high expression of miR-21 was associated with shorter survival time compared with low expression(P＜0.05).Conclusion:miR-21 participate in the process of colon cancer by regulating several target genes.The expression of miR-21 was increased in colon patients compared with that in healthy controls，and high expression of miR-21 indicates a poor prognosis of colon cancer patients. |
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| Keywords: | colon cancer miR-21 function analysis prognosis analysis |
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