| RP-HPLC法同时测定畅鼻通颗粒中3种组分的含量 |
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| 引用本文： | 申 涛,徐海燕,姜 煜,栾 爽,赵怀清. RP-HPLC法同时测定畅鼻通颗粒中3种组分的含量[J]. 沈阳药科大学学报, 2008, 25(8): 642-645 |
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| 作者姓名： | 申 涛 徐海燕 姜 煜 栾 爽 赵怀清 |
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| 作者单位： | [1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学高等职业技术学院,辽宁沈阳110026 |
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| 摘 要： | 目的建立同时测定畅鼻通颗粒中桂皮酸、黄芩苷和黄芩素含量的反相高效液相色谱法。方法采用反相高效液相色谱法进行含量测定。色谱柱为DiamonsilTMC18(4.6 mm×200 mm,5μm)色谱柱,流动相为甲醇-乙腈-体积分数为1%的冰醋酸水溶液(体积比为65∶20∶115),流速为1.0 mL.min-1,检测波长为280 nm。结果桂皮酸在0.82~8.20 mg.L-1内,黄芩苷在31.00~310.00 mg.L-1内,黄芩素在3.20~32.00 mg.L-1内,质量浓度与峰面积线性关系良好,相关系数分别为0.999 9、0.999 9和0.999 7;平均回收率分别为101.1%、100.5%和101.4%;精密度RSD均小于2.0%。结论RP-HPLC法可用于畅鼻通颗粒的质量控制。
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| 关 键 词： | 畅鼻通颗粒 桂皮酸 黄芩苷 黄芩素 高效液相色谱法 |
| 收稿时间： | 2007-10-16 |
Simultaneous determination of three components in Changbitong granules by RP-HPLC |
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| SHEN Tao,XU Hai-yang,JIANG Yu,LUAN Shuang,ZHAO Huai-qing. Simultaneous determination of three components in Changbitong granules by RP-HPLC[J]. Journal of Shenyang Pharmaceutical University, 2008, 25(8): 642-645 |
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| Authors: | SHEN Tao XU Hai-yang JIANG Yu LUAN Shuang ZHAO Huai-qing |
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| Affiliation: | 1.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2.School of vocational technical,Shenyang Pharmaceutical University, Shenyang 110026, China |
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| Abstract: | Objective To develop a RP-HPLC method to simultaneously determine the concentrations of cinnamic acid, baicalin and baicalein in Changbitong(tradition Chinese medicines) granules. Method The separation was performed on a DiamonsilTM C18 (4.6 mm×200 mm, 5 μm) column with a mixture of methnol-acetonitrile-1% acetic acid solution (V: V: V=65: 20: 115) as mobile phase. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 280nm. Results A good linearity was obtained within the range of 0.82-8.20 mg·L-1（r=0.999 9） for cinnamic acid,31.00-310.00 mg·L-1（r=0.999 9）for baicalin and 3.20-32.00 mg·L-1(r=0.999 7) for baicalein. The average recoveries of the three components were 101.1%, 100.5% and 101.4%, respectively. The precision was not more than 2.0%. Conclusion The method is proved to be selective and reproducible; it is suitable for the quality control of Changbitong granules. |
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| Keywords: | Changbitong granules')" >Changbitong granules cinnamic acid baicalin baicalein ')" >RP-HPLC |
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