首页 | 本学科首页   官方微博 | 高级检索  
     

不同形貌纯钛表面对人脐静脉内皮细胞生物学行为的影响
引用本文:梁乃文,石磊,黄颖,邓旭亮. 不同形貌纯钛表面对人脐静脉内皮细胞生物学行为的影响[J]. 北京大学学报(医学版), 2017, 49(1): 43-048. DOI: 10.3969/j.issn.1671-167X.2017.01.007
作者姓名:梁乃文  石磊  黄颖  邓旭亮
作者单位:北京大学口腔医学院·口腔医院修复科,北京100081;北京大学口腔医学院·口腔医院特诊科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室,北京100081;北京大学口腔医学院·口腔医院修复科,北京100081;北京大学口腔医学院·口腔医院特诊科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室,北京100081
基金项目:国家自然科学基金(81171000;81400563)资助 Supported by the National Natural Sciences Foundation of China
摘    要:
目的: 以人脐静脉内皮细胞(human umbilical vein endothelial cell, HUVECs)为模型细胞,研究纯钛表面的形貌因素对种植体周围成血管效应的可能影响。方法: 构建不同结构形貌的纯钛表面,包括光滑表面、纳米结构表面、微米结构表面以及微米/纳米复合结构表面,检测不同形貌纯钛表面对HUVECs的黏附和增殖能力,检测血管内皮生长因子(vascular endothelial growth factor, VEGF)的分泌能力和HUVECs内钙离子(calcium,Ca2+)的浓度。结果: 不同形貌纯钛材料在扫描电子显微镜下分别具有光滑表面、纳米结构表面、纳米结构表面和微米/纳米复合结构表面。扫描电子显微镜和细胞骨架染色结果显示,在不同形貌纯钛表面孵育24 h后,光滑表面结构组的细胞数目从(18±4)个/视野增加到(42±6)个/视野,纳米结构表面组的细胞数目从(28±6)个/视野增加到(52±10)个/视野,微米结构表面组的细胞数目从(20±4)个/视野增加到(21±6)个/视野,微米/纳米复合结构组表面的细胞数目从(16±4)个/视野增加到(18±6)个/视野,微米结构表面组和微米/纳米复合结构表面组黏附和增殖的细胞数目显著少于光滑表面结构组和纳米结构表面组(P<0.05)。酶联免疫吸附定量研究显示,HUVECs在光滑结构表面、纳米结构表面、微米结构表面以及微米/纳米复合结构表面VEGF分泌量分别为(690±35) ng/L、(560±20) ng/L、(474±43) ng/L、(517±29) ng/L,与光滑结构表面相比,HUVECs在微米结构表面和微米/纳米复合结构表面分泌的VEGF量显著减少(P<0.05)。Ca2+免疫荧光探针检测结果显示,HUVECs细胞内Ca2+在微米结构表面和微米/纳米复合结构表面的表达显著高于在光滑结构表面和纳米结构表面。结论: HUVECs在微米结构表面和微米/纳米复合结构表面的功能受影响可能与在这些表面HUVECs内钙浓度的增加相关,因此,种植体表面的形貌因素可能通过影响内皮细胞的功能,对种植体周围的成血管效应造成影响。

关 键 词:牙种植体    表面形貌  人脐静脉内皮细胞  

Role of different scale structures of titanium implant in the biological behaviors of human umbilical vein endothelial cells
LIANG Nai-wen,SHI Lei,HUANG Ying,DENG Xu-liang. Role of different scale structures of titanium implant in the biological behaviors of human umbilical vein endothelial cells[J]. Journal of Peking University. Health sciences, 2017, 49(1): 43-048. DOI: 10.3969/j.issn.1671-167X.2017.01.007
Authors:LIANG Nai-wen  SHI Lei  HUANG Ying  DENG Xu-liang
Affiliation:(1.Department of Prosthodontics, 2.Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China)
Abstract:
Objective:To study the role of different scale structure of Ti implants on the biological behaviors of human umbilical vein endothelial cell (HUVECs) and to reveal the role of material surface topographical features on peri-implant angiogenesis.Methods:Titanium(Ti) discs with different surface structures (Ti discs with smooth surface,Ti discs with nano scale structure,Ti discs with micro scale structure and Ti discs with micro/nano scale structure,named as SM-Ti,Nano-Ti,Micro-Ti and Micro/ nano-Ti,respectively) were prepared and their surface topographical features were confirmed via scanning electron microscopy (SEM) observation.HUVECs were cultured on these Ti discs.Biological outcomes of HUVECs on different surfaces were carried out,including cell adhesive capacity,proliferation,vascular endothelial growth factor (VEGF) production and intracellular expression of Ca2+.Results:The results of SEM images and immunofluorescence double staining of rhodamine-phalloidin and DAPI showed that compared with the SM-Ti and Nano-Ti group,the adhesive capacity and proliferation behavior of HUVECs on the surfaces of Micro-Ti and Micro/nano-Ti was decreased.The results of culturing HUVECs on different groups of Ti discs after 24 hours showed that the cells number grew from (18 4) to (42 ± 6)/vision on SM-Ti,(28 ±6) to (52 ± 10)/vision on Nano-Ti,(20 ±4) to (21 ±6)/vision on MicroTi and (16 ±4) to (18 ±6)/vision on Micro/nano-Ti.Moreover,compared with the adhesion and proliferation of HUVECs on SM-Ti group and Nano-Ti,the adhesion and proliferation of HUVECs on MicroTi group and Micro/nano-Ti group was significantly reduced (P < 0.05).The results of enzyme-linked immunosorbent assay (ELISA) showed that the VEGF productions of SM-Ti,Nano-Ti,Micro-Ti and Micro/nano-Ti were (690 ±35) ng/L,(560 ±20) ng/L,(474 ±43) ng/L and (517 ±29) ng/L,respectively.Moreover,compared with the VEGF production of HUVECs on SM-Ti group,the VEGF production of HUVECs on Micro-Ti group and Micro/nano-Ti group was significantly reduced (P <0.05).The results of Ca2 + ion detection showed that the Ca2 + expression of HUVECs on Micro-Ti and Micro/nano-Ti was significantly higher than that on the surface of SM-Ti and Nano-Ti.These results implied that the over expressed Ca2+ might contributed to the impaired biological function of HUVECs on Micro-Ti and Micro/nano-Ti.Conclusion:Different topographical features on titanium influenced the biological behaviors of the HUVECs,which may illustrate how topographical features of Ti implant affect peri-implant angiogenesis.These results also suggest that the biological behaviors of HUVECs might be relative to the changed expression of intracellular Ca2+.
Keywords:Dental implants,titanium  Surface topographical feature  Human umbilical vein endothelial cell  Calcium
本文献已被 万方数据 等数据库收录!
点击此处可从《北京大学学报(医学版)》浏览原始摘要信息
点击此处可从《北京大学学报(医学版)》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号