Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay. |
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| Authors: | P Sch fer, W Tenschert, K Gutensohn, R Laufs |
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| Affiliation: | P Schäfer, W Tenschert, K Gutensohn, and R Laufs |
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| Abstract: | Quantitative cytomegalovirus antigenemia and DNAemia were determined in peripheral leukocytes of 25 patients stored for up to 72 h at room temperature (RT) and 4 degrees C before processing. Numbers of antigen-positive cells significantly decreased with time. The decline was greater at RT than at 4 degrees C. In contrast, no significant alterations in DNAemia occurred. |
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