Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei |
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| Authors: | U'Ren Jana M Van Ert Matthew N Schupp James M Easterday W Ryan Simonson Tatum S Okinaka Richard T Pearson Talima Keim Paul |
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| Affiliation: | Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640, USA. |
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| Abstract: | A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia. |
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