| 胰岛素样生长因子1及血小板源性生长因子对人退变髓核细胞生物学活性的影响 |
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| 引用本文： | 林 旺,刘寿坤,王盈盈,郭卫中,林成寿,王 旭,苏 郁. 胰岛素样生长因子1及血小板源性生长因子对人退变髓核细胞生物学活性的影响[J]. 中国组织工程研究, 2012, 16(2): 223-226. DOI: 10.3969/j.issn.1673-8225.2012.02.007 |
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| 作者姓名： | 林 旺 刘寿坤 王盈盈 郭卫中 林成寿 王 旭 苏 郁 |
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| 作者单位： | 福建医科大学附属宁德市闽东医院,福建省福安市 355002 |
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| 摘 要： | 背景：有研究表明胰岛素样生长因子1和血小板源性生长因子可抑制人椎间盘细胞凋亡。目的：观察在体外培养条件下胰岛素样生长因子1、血小板源性生长因子对人退变髓核细胞生物学活性的影响。方法：体外单层培养人退变髓核细胞,通过免疫组织化学鉴定细胞。对传3代人退变髓核细胞采用分别不同生长因子干预,实验分4组：胰岛素样生长因子1组,血小板源性生长因子组,胰岛素样生长因子1+血小板源性生长因子组及对照组。 结果与结论：胰岛素样生长因子1、血小板源性生长因子均可促进细胞增殖,促进细胞合成Ⅱ型胶原和聚集蛋白聚糖,其中胰岛素样生长因子1促Ⅱ型胶原合成作用强于血小板源性生长因子(P < 0.05),血小板源性生长因子促蛋白聚糖合成作用强于胰岛素样生长因子1(P < 0.05)。胰岛素样生长因子1促进细胞合成Ⅰ型胶原,血小板源性生长因子抑制细胞合成Ⅰ型胶原。结果证实,胰岛素样生长因子1、血小板源性生长因子均可通过促进细胞增殖、促进细胞合成Ⅱ型胶原和聚集蛋白聚糖,从而提高人退变髓核细胞的生物学活性。
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| 关 键 词： | 胰岛素样生长因子1 血小板源性生长因子 髓核细胞 退变 组织工程 |
| 收稿时间： | 2011-08-19 |
Effect of insulin-like growth factor-1 and platelet-derived growth factor on the biologicalpotential of human degenerative nucleus pulposus cells |
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| Lin Wang,Liu Shou-kun,Wang Ying-ying,Guo Wei-zhong,Lin Cheng-shou,Wang Xu,Su Yu. Effect of insulin-like growth factor-1 and platelet-derived growth factor on the biologicalpotential of human degenerative nucleus pulposus cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(2): 223-226. DOI: 10.3969/j.issn.1673-8225.2012.02.007 |
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| Authors: | Lin Wang Liu Shou-kun Wang Ying-ying Guo Wei-zhong Lin Cheng-shou Wang Xu Su Yu |
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| Affiliation: | Mindong HospitalAffiliated to FujianMedical University,Fuan 355002,Fujian Province,China
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| Abstract: | BACKGROUND:Previous studies showed that insulin-like growth factor-1 (IGF-1) and platelet-derived growth factor (PDGF) cansuppress apoptosis of human intervertebral cells.OBJECTIVE:To observe the effects of IGF-1 and PDGF on the biological potential of the human degenerative NP cells in vitro.METHODS:Human degenerative nucleus pulposus cells were cultured in monolayer in vitro and identified byimmunohistochemical staining. The cells of passage 3 were treated separately with different growth factors and then randomlydivided into 4 groups: IGF-1 group, PDGF group, IGF-1+PDGF group and control group.RESULTS AND CONCLUSION:IGF-1 and PDGF could stimulate cell proliferation and synthesis of collagen type Ⅱ andaggrecan, and IGF-1 raised more content of collagen typeⅡ than the PDGF (P < 0.05), but the PDGF raised more content ofaggrecan than the IGF-1 (P < 0.05). IGF-1 stimulated the synthesis of collagen typeⅠ, then PDGF inhibited the synthesis. Boththe IGF-1 and PDGF can stimulate cell proliferation, the synthesis of collagen type Ⅱ and aggrecan, thereby enhance thebiological potential of the human degenerative nucleus pulposus cells. |
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