| miR-217调控lncRNAMALAT1影响食管鳞癌细胞的生物学行为 |
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| 引用本文： | 徐健,江跃全,蔡华荣,尹哲,张奇. miR-217调控lncRNAMALAT1影响食管鳞癌细胞的生物学行为[J]. 中国临床解剖学杂志, 2018, 36(4): 408-413. DOI: 10.13418/j.issn.1001-165x.2018.04.011 |
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| 作者姓名： | 徐健 江跃全 蔡华荣 尹哲 张奇 |
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| 作者单位： | 重庆大学附属肿瘤医院肿瘤转移与个体化诊治转化研究重庆市重点实验室， 重庆 400030 |
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| 基金项目： | 重庆市卫生局资助项目（2013-2-122 ） |
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| 摘 要： | 目的 探讨miR-217通过调控lncRNA MALAT1抑制食管鳞状细胞癌细胞的增殖，迁移和侵袭行为及其机制。 方法 qPCR检测miR-217在食管鳞状细胞癌组织和不同细胞株中的表达情况；双荧光素酶报告基因检测miR-217与MALAT1之间的相互作用；MTT增殖实验检测抑制miR-217后食管鳞状细胞癌细胞的增殖能力的变化情况；划痕愈合试验和Transwell侵袭实验检测抑制miR-217后食管鳞状细胞癌细胞的迁移和侵袭行为的变化情况；Western blotting实验检测miR-217对MALAT1下游相关蛋白表达情况的影响。 结果 与正常食管组织相比，食管鳞状细胞癌组织中miR-217的表达水平相对上调，与其他细胞株相比，Ec109细胞中miR-217表达最高；双荧光素酶实验证实miR-217能与MALAT1的3’ UTR特异性结合，可以调控MALAT1的表达与活性；抑制miR-217的表达后可以抑制食管鳞状细胞癌细胞的增殖、迁移和侵袭能力；抑制miR-217后MALAT1下游MIA2，ROBO1表达明显下调。 结论 miR-217可以调控MALAT1的表达影响食管鳞状细胞癌细胞的生物学行为。
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| 关 键 词： | MALAT1 食管鳞状细胞癌 miR-217 MIA2 |
| 收稿时间： | 2018-04-25 |
miR-217 inhibits proliferation,migration, and invasion of esophageal squamous cell carcinoma cells by regulating lncRNA MALAT1 |
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| XU Jian,JIANG Yue-quan,CAI Hua-rong,YIN Zhe,ZHANG Qi. miR-217 inhibits proliferation,migration, and invasion of esophageal squamous cell carcinoma cells by regulating lncRNA MALAT1[J]. Chinese Journal of Clinical Anatomy, 2018, 36(4): 408-413. DOI: 10.13418/j.issn.1001-165x.2018.04.011 |
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| Authors: | XU Jian JIANG Yue-quan CAI Hua-rong YIN Zhe ZHANG Qi |
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| Affiliation: | Chongqing University Cancer Hospital, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing 400300，China |
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| Abstract: | Objective To investigate the inhibitory effect of miR-217 on proliferation, migration and invasion of esophageal squamous cell carcinoma cells by regulating lncRNA MALAT1. Methods qPCR was used to detect the expression of miR-217 in esophageal squamous cell carcinoma and different cell lines. Dual luciferase reporter assay detected the interaction between miR-217 and MALAT1. MTT assay was used to detect the proliferation of esophageal squamous cell carcinoma cells after miR-217 was inhibited. Scratch healing assay and Transwell invasion assay were used to detect the migration and invasion of esophageal squamous carcinoma cells after miR-217 was inhibited. The effect of miR-217 on the expression of MALAT1 downstream protein was detected by Western blotting. Results Compared with normal esophageal tissue, the expression of miR-217 in esophageal squamous cell carcinoma was relatively up-regulated, and the expression of miR-217 was the highest in Ec109 cells compared with other cell lines. Dual luciferase assay confirmed that miR-217 could specifically bind to 3'UTR of MALAT1 and regulate the expression and activity of MALAT1. Inhibition of miR-217 expression could inhibit esophageal squamous cell carcinoma cell proliferation, migration and invasion ability. Down-regulation of miR-217 after MALAT1 MIA2, ROBO1 expression was significantly down-regulated. Conclusion miR-217 can regulate the expression of MALAT1 and affect the biological behavior of esophageal squamous cell carcinoma cells. |
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| Keywords: | MALAT1 Esophageal squamous cell carcinoma miR-217 mIA2 |
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