MicroRNA-188-5p targeting Forkhead Box L1 promotes colorectal cancer progression via activating Wnt/β-catenin signaling |
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| Authors: | JIALIN WU ZEHONG CHEN WENWEI LIU YONGXIN ZHANG WEI FENG YUJIE YUAN JINNING YE LIANG WANG SHIRONG CAI YULONG HE SUIJING WU WU SONG |
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| Affiliation: | 1 Center of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China2 Department of Hepatobiliary and Pancreatic Surgery, Union Shenzhen Hospital, Huazhong University of Science and Technology, Shenzhen, China3 Department of Gastrointestinal Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China4 Department of Hematology, Guangdong General Hospital, Guangzhou, China |
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| Abstract: | Objective: MicroRNA-188-5p (miR-188) enhances oncologic progression in various human malignancies. Thisstudy aimed to explore its role in colorectal cancer (CRC). Materials and Methods: Human CRC tissues paired withnormal tissues, and several CRC cell lines were utilized. Real-time quantitative PCR was applied to measure theexpression of miR-188. Overexpression and knockdown were used to access the function of miR-188 and toinvestigate whether FOXL1/Wnt signaling mediates such function. The proliferation, migration and invasion of cancercells were evaluated by CCK8, wound-healing and transwell assays, respectively. Whether FOXL1 acted as a directtarget of miR-188 was verified by dual-luciferase reporter assays. Results: Levels of miR-188 were upregulated in CRCtissues than in paired-normal tissues, as well as in various CRC cell lines. High expression of miR-188 was stronglyassociated with advanced tumor stage, accompanied with significant tumor cell proliferation, invasion and migration.It was confirmed that FOXL1 played positive crosstalk between miR-188 regulation and downstream Wnt/β-cateninsignaling activation. Conclusions: All findings indicate that miR-188 promotes CRC cell proliferation and invasionthrough targeting FOXL1/Wnt signaling and could be served as a potential therapeutic target for human CRC in thefuture. |
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| Keywords: | miR-188 Colorectal adenocarcinoma Oncogenesis Oncologic progression Wnt signaling |
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