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| 微生物验证中标准菌株菌悬液制备新方法的探讨 | |
| 引用本文： | 王蕴红 谢文麟 梁雪. 微生物验证中标准菌株菌悬液制备新方法的探讨[J]. 国际生物制品学杂志, 2021, 44(2): 93-96. DOI: 10.3760/cma.j.cn311962-20200603-00062 |
| 作者姓名： | 王蕴红 谢文麟 梁雪 |
| 作者单位： | 成都蓉生药业有限责任公司质量检定部 610041 |
| 摘 要： | 目的:探讨采用复苏等量分装的标准贮备菌株培养18～24 h后配制的菌悬液作为生物制品微生物验证用菌液的可行性。方法:制备等量分装的标准贮备菌株，选择保藏0、3、6、9、12、13个月对其复苏后菌液进行菌落计数，对计数结果进行单因素方差分析。结果:分析显示，储存3、6、9、12、13个月的金黄色葡萄球菌、大肠埃希菌、铜绿... |
| 关 键 词： | 集落计数，微生物 模式培养物 菌株 悬液 |
Discussion on a new preparing method of standard bacterium suspension in microbial verification#br# |
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| Affiliation: | Quality Control Department, Chengdu Rongsheng Pharmaceuticals Co., Ltd., Chengdu 610041, China |
| Abstract: | Objective To evaluate the feasibility of preparing bacterium suspension by recovering the standard bacterium in equal volume and culturing 18-24 h for microbial verification. Methods Standard bacteria were prepared in equal volume. Then the recovered strains were counted after storing for 0, 3, 6, 9, 12, 13 months. The colony counting results were analyzed by single-factor analysis of variance. Results Compared with 0 month, the counts of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans after storing for 3, 6, 9, 12, 13 months were stable and of no statistically significant difference, with F values of 2.24, 2.21, 0.58 and 1.59, respectively, and all P values >0.05. Conclusion Using recovered standard bacterium in equal volume to prepare the bacterium suspension for microbial verification of biological products is feasible,and with bacterium concentration stable . |
| Keywords: | Colony count microbial Type culture Bacterium strain Suspension |
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