Sfh1, an essential component of the RSC chromatin remodeling complex,maintains genome integrity in fission yeast |
| |
| Authors: | Masahiro Kuragano Takahiro Asanuma Hiromi Nakagawa Katsunori Tanaka Yota Murakami |
| |
| Affiliation: | 1. Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Japan;2. School of Medicine, Oita University, Yufu, Japan;3. Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Japan;4. Laboratory of Cell Regulation, Department of Cell Biology, Institute for Virus Research, Kyoto University, Kyoto, Japan;5. Laboratory of Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Japan |
| |
| Abstract: | Abp1 is a fission yeast CENP‐B homologue that contributes to centromere function, silencing at pericentromeric heterochromatin and silencing of retrotransposons. We identified the sfh1 gene, encoding a core subunit of the fission yeast chromatin remodeling complex RSC as an Abp1‐interacting protein. Because sfh1 is essential for growth, we isolated temperature‐sensitive sfh1 mutants. These mutants showed defects in centromere functions, reflected by sensitivity to an inhibitor of spindle formation and minichromosome instability. Sfh1 localized at both kinetochore and pericentromeric heterochromatin regions. Although sfh1 mutations had minor effect on silencing at these regions, they decreased the levels of cohesin on centromeric heterochromatin. Sfh1 also localized at a retrotransposon, Tf2, in a partly Abp1‐dependent manner, and assisted in silencing of Tf2 by Abp1 probably in the same pathway as a histone chaperon, HIRA, which is also known to involve in Tf2 repression. Furthermore, sfh1 mutants were sensitive to several DNA‐damaging treatments (HU, MMS, UV and X‐ray). Increase in spontaneous foci of Rad22, a recombination Mediator protein Rad52 homologue, in sfh1 mutant suggests that RSC functions in homologous recombination repair of double‐stranded break downstream of the Rad22 recruitment. These results indicate that RSC plays multiple roles in the maintenance of genome integrity. |
| |
| Keywords: | |
|
|
