Flotillin-2 is an acrosome-related protein involved in mouse spermiogenesis |
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| Authors: | Yibo Wu Xin Chen Shuai Wang Min Jiang Bo Zheng Quan Zhou Ye Bi Zuomin Zhou Xiaoyan Huang Jiahao Sha |
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| Affiliation: | State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, Jiangsu 210029, China |
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| Abstract: | Spermatogenesis is a complex process of terminal differentiation by which mature sperms are generated, and it can be divided into three phases: mitosis, meiosis and spermiogenesis. In a previous study, we established a series of proteomic profiles for spermatogenesis to understand the regulation of male fertility and infertility. Here, we further investigated the localization and the role of flotillin-2 in spermiogenesis. Flotillin-2 expression was inves-tigated in the testis of male CD1 mice at various developmental stages of spermatogenesis by using Western blot-ting, immunohistochemistry and immunofluorescence. Flotillin-2 was knocked down in vivo in three-week-old male mice using intratesticular injection of small inhibitory RNA (siRNA), and sperm abnormalities were assessed three weeks later. Flotillin-2 was expressed at high levels in male germ cells during spermatogenesis. Flotillin-2 immunoreactivity was observed in pachytene spermatocytes as a strong dot-shaped signal and in round spermatids as a sickle-shaped distribution ahead of the acrosome. Immunofluorescence confirmed flotillin-2 was localized in front of the acrosome in round spermatids, indicating that flotillin-2 was localized to the Golgi apparatus. Knock-down of flotillin-2 in vivo led to a significant increase in head sperm abnormalities isolated from the cauda epidi-dymis, compared with control siRNA-injected testes. This study indicates that flotillin-2 is a novel Golgi-related protein involved in sperm acrosome biogenesis |
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| Keywords: | flotillin-2 Golgi apparatus RNA interference acrosome biogenesis |
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