Karyotyping of Single Human Chromosomes from Dry Mass Determined by Electron Microscopy |
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| Authors: | Gunter F. Bahr and Harvey M. Golomb |
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| Affiliation: | *Armed Forces Institute of Pathology, Washington, D.C. 20305;†Department of Pathology, Georgetown University, Washington, D.C. 20007 |
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| Abstract: | Human chromosomes from cultures of peripheral lymphocytes were prepared for electron microscopy by surface spreading and critical-point drying. Total and partial determinations of chromosomal dry mass were made from contrast in electron microscopic negatives. The distribution of dry weights was analyzed with respect to the Denver-Chicago classifications. There is considerable variability of mass within each group, but no statistically significant differences between four donors. A single chromosome can be correctly classified from its total mass and the ratio of long- to short-arm mass. |
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