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Initial research of screening for the differentially expressed proteins in beagles irradiated with 4.5 Gy60 CO γ-rays by two-dimensional gel electrophoresis and mass spectrometry
作者姓名：谢玲  李明  邢爽  黄海潇  欧红玲  熊国林  赵燕芳  赵振虎  王宁  王金香  缪竞诚  朱南康  张学光  从玉文  罗庆良
作者单位：军事医学科学院放射与辐射研究所,北京,100850;第二炮兵总医院;苏州大学医学部;
基金项目：国防科工委"十一五"核科学基础研究重点项目
摘    要：Objective To explore the mechanisms of cytokines on acute radiation disease in irradiated beagles.Methods The sera of beagles irradiated with 4.5 Gy γ-rays with cytokines treatment was collected at different time points post irradiation.The two-dimensional gel electrophoresis(2-DE)was used to isolate and compare the differentially expressed proteins in sera.HD-MS was used to analyze the differentially expressed proteins with significance,and the amino acid sequences should be determined. Results High resolution 2-DE gel map was obtained.There were six differentially expressed proteins in sera of irradiated beagles at different time points.Four protein spots were successfully identified by MS.A significant spot was identified as serum amyloid A(SAA)by HD-MS,with relative molecular mass of 13 077 and isoelectfie point of 6.26.Expression of SAA was not found 1 d pre-irradiation and 36 d postirradiation,but increased slightly 1 d(0.2166)and significantly 14 d post-irradiation(0.4577). Conclusions The expression of serum amyloid A was consistent with the process of acute radiation injury,which might indicate the turnover of the disease.
关 键 词：急性放射损伤   双向电泳   高解析电喷雾串联质谱仪   血清淀粉样蛋白A   
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