Maintenance of CD8+ memory T lymphocytes in the spleen but not in the bone marrow is dependent on proliferation |
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| Authors: | Francesco Siracusa Özen Sercan Alp Patrick Maschmeyer Mairi McGrath Mir‐Farzin Mashreghi Shintaro Hojyo Hyun‐Dong Chang Koji Tokoyoda Andreas Radbruch |
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| Affiliation: | 1. German Rheumatism Research Center (DRFZ), Department of Cell Biology, Institute of the Leibniz Association, Berlin, Germany;2. German Rheumatism Research Center (DRFZ), Department of Osteoimmunology, Institute of the Leibniz Association, Berlin, Germany;3. Charité University Medicine Berlin, Berlin, Germany |
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| Abstract: | It is current belief that numbers of CD8+ memory T lymphocytes in the memory phase of an immune response are maintained by homeostatic proliferation. Here, we compare the proliferation of CD8+ memory T lymphocytes, generated by natural infections and by intentional immunization, in spleen and bone marrow (BM). Fifty percent of CD8+ memory T lymphocytes in the spleen are eliminated by cyclophosphamide within 14 days, indicating that numbers of at least 50% of splenic CD8+ memory T lymphocytes are maintained by proliferation. The numbers of CD8+ memory T lymphocytes in the BM, however, were not affected by cyclophosphamide. This stability was independent of circulating CD8+ memory T cells, blocked by FTY720, showing that BM is a privileged site for the maintenance of memory T lymphocytes, as resident cells, resting in terms of proliferation. |
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| Keywords: | Bone marrow CD8+ memory T lymphocytes Cyclophosphamide Homeostatic proliferation Tissue resident memory |
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