| 兔VX2舌鳞癌移植瘤模型3种建立方法的比较 |
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| 引用本文： | 田军,廖贵清,刘海潮,李传真,李清,王敬旭,杜日昌,张同韩. 兔VX2舌鳞癌移植瘤模型3种建立方法的比较[J]. 华西口腔医学杂志, 2009, 27(3): 326-329 |
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| 作者姓名： | 田军 廖贵清 刘海潮 李传真 李清 王敬旭 杜日昌 张同韩 |
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| 作者单位： | 1.中山大学附属口腔医院口腔颌面外科， 广东广州510055；2.广东省韶关市第一人民医院病理科， 广东韶关512000 |
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| 基金项目： | 国家自然科学基金资助项目，广东省自然科学基金资助项目 |
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| 摘 要： | 目的采用3种不同移植瘤方法建立稳定的兔VX2舌鳞癌移植瘤模型，并对这3种方法建立的模型的生物学特性进行比较。方法将72只新西兰大白兔随机分成3组，每组24只，分别用瘤组织块植入法、改良瘤细胞悬液注入法及瘤细胞悬液注入法植于兔舌侧缘中1/3黏膜下，建立兔舌鳞癌的移植瘤模型。观察和比较3种方法所建立的模型的肿瘤生长状况、成瘤率和自发转移发生率。结果瘤组织块植入组、改良瘤细胞悬液注入组、瘤细胞悬液注入组的成瘤率分别为83.3%、91.7%、33.3%，局部淋巴结转移率分别为71.4%、100.0%、37.5%，肺转移率分别为35.7%、81.3%、0；3种方法建立的兔VX2舌鳞癌移植瘤模型的组织学形态均符合中分化鳞状细胞癌特征。结论3种方法建立的移植瘤模型均较接近人舌鳞癌自然生长过程。其中，改良瘤细胞悬液注入法建立的动物模型，因成瘤率高、转移率高、同期瘤体大小差异较小，更适合于舌鳞癌研究。
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| 关 键 词： | 舌癌 VX2肿瘤细胞株 动物模型 |
| 收稿时间： | 2009-06-25 |
| 修稿时间： | 2009-06-25 |
A comparison of three methods in establishing transplanted model of VX2 tongue carcinoma in rabbits |
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| TIAN Jun,LIAO Gui-qing,LIU Hai-chao,LI Chuan-zhen,LI Qing,WANG Jing-xu,DU Ri-chang,ZHANG Tong-han. A comparison of three methods in establishing transplanted model of VX2 tongue carcinoma in rabbits[J]. West China journal of stomatology, 2009, 27(3): 326-329 |
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| Authors: | TIAN Jun LIAO Gui-qing LIU Hai-chao LI Chuan-zhen LI Qing WANG Jing-xu DU Ri-chang ZHANG Tong-han |
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| Affiliation: | 1. Dept. of Oral and Maxillofacial Surgery，Stomatological Hospital，Sun Yat-Sen University, Guangzhou 510055, China; 2. Dept. of Pathology, Shaoguan Frist People′s Hospital, Shaoguan 512000, China |
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| Abstract: | Objective To establish transplanted models of VX2 tongue carcinoma in rabbits by three methods and compare these models. Methods After establishment of VX2 tumor-bearing rabbits, 72 New-Zealand white rabbits were randomly divided into 3 groups. Intact tumour tissue, modified tumour cell suspension, tumour cell suspension were respectively injected into the middle-third lateral border of the tongues of rabbits in 3 groups to induce transplanted VX2 tongue carcinoma. The histological features, the tumour -take rates and the metastasis rates of the 3 models were observed. Results The tumour-take rate of 3 models were 83.3%, 91.7% and 33.3% respectively; the lymph node metastasis rates were 71.4%, 100.0% and 37.5% respectively; the lung metastasis rates were 35.7%, 81.3% and 0 respectively. The histological features of the transplanted VX2 tongue carcinoma of 3 models were all consistent with those of moderately differentiated carcinoma. Conclusion The biological properties of the transplanted VX2 tongue carcinoma of 3 models is much alike to tongue carcinoma in humans. The model established with modified tumor cell suspension is considered to be more suitable for tongue cancer study. |
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| Keywords: | tongue carcinoma VX2 tumor cell strain animal model |
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