Hapten-specific T-Cell Unresponsiveness Induced by Benzylpenicilloyl Autologous Gamma Globulin Conjugates in Human Lymphocytes In Vitro |
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| Authors: | Raif S. Geha Lazar Fruchter Yves Borel |
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| Affiliation: | Division of Allergy, Department of Medicine, Children''s Hospital Medical Center, Boston, Massachusetts 02115;Division of Immunology, Department of Medicine, Children''s Hospital Medical Center, Boston, Massachusetts 02115;Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115 |
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| Abstract: | The aim of these studies was to determine whether unresponsiveness to the main determinant of penicillin, benzylpenicilloyl, can be induced in human peripheral lymphocytes in vitro by conjugates of benzylpenicilloyl (BPO) autologous gamma globulin (HGG). Initially it was shown that conjugates of BPO-keyhole limpet hemocyanin (KLH) elicited lymphocyte proliferation in the peripheral blood lymphocytes of six out of nine adult individuals in vitro. In contrast, conjugates of dinitrophenylated KLH and of BPO-HGG and the carriers HGG and KLH alone failed to do so. Similarly, release of the non-specific helper factor, lymphocyte mitogenic factor (LMF) occurred only after BPO-KLH stimulation. LMF activity was measured by B-cell proliferation and incorporation of radioactive amino acids into secreted immunoglobulin. |
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