| 口腔鳞癌组织hMSH2外显子突变筛选的初步研究 |
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| 引用本文： | 程斌,夏鸿彬,李春阳,洪筠,杨灵澜. 口腔鳞癌组织hMSH2外显子突变筛选的初步研究[J]. 华西口腔医学杂志, 2002, 20(2): 144-146 |
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| 作者姓名： | 程斌 夏鸿彬 李春阳 洪筠 杨灵澜 |
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| 作者单位： | 510060 中山大学光华口腔医学院 |
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| 基金项目： | 国家自然科学基金 (编号 3 970 0 15 9)，广东省卫生厅基金 (编号A19982 3 1) |
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| 摘 要： | 目的:探讨错配修复基因hMSH2在口腔鳞癌发生发展中的作用。方法:应用PCR-SSCP法检测30例口腔粘膜鳞状细胞癌组织标本中hMSH2外显子5、6、7、8、9、12的突变情况。结果:在30例口腔鳞癌中,hMSH2六个外显子 PCR均扩增到目的DNA片段;SSCP检测未检出hMSH2六个外显子发生突变。结论:口腔鳞癌hMSH2外显子结构改变模式与遗传性肿瘤不同;错配修复基因在口腔鳞癌发生发展中的作用值得探讨。
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| 关 键 词： | 口腔鳞状细胞癌 错配修复基因 hMSH2 突变 |
| 收稿时间： | 2002-04-25 |
| 修稿时间： | 2001-12-29 |
The Detection of Exon Mutation of hMSH2 in Tissues of Oral Squamous Cell Carcinoma |
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| Cheng Bin,Xia Hongbin,Li Chunyang,et al,Guanghua College of Stomatology,Sun Yat_Sen University,. The Detection of Exon Mutation of hMSH2 in Tissues of Oral Squamous Cell Carcinoma[J]. West China journal of stomatology, 2002, 20(2): 144-146 |
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| Authors: | Cheng Bin Xia Hongbin Li Chunyang et al Guanghua College of Stomatology Sun Yat_Sen University |
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| Affiliation: | Guanghua College of Stomatology,Sun Yat-Sen University |
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| Abstract: | Objective:The purpose of this study was to detect the exon mutation of mismatch repair gene hMSH2 in tissues of oral squamous cell carcinoma (OSCC) and investigate the role of hMSH2 in the pathogenesis of OSCC.Methods:The mutations of hMSH2 exon 5, 6, 7, 8, 9 and 12 in the tissues of 30 human OSCC cases were analyzed using PCR_SSCP.Results:The six exons of hMSH2 were amplified successfully by using PCR technique, while no mutations in these exons were identified by using PCR_SSCP.Conclusion:The structure changes of hMSH2 exons in OSCC may be different from that in hereditary non_polyposis colorectal carcinoma (HNPCC); the role of hMSH2 in carcinogenesis of OSCC needs to be evaluated further.; |
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| Keywords: | oral squamous cell carcinoma mismatch repair hMsh2 mutation |
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