| 寡核苷酸YW002对人牙周膜干细胞增殖、周期、凋亡和早期成骨分化的影响 |
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| 引用本文： | 毕雪婷,申玉芹,徐晓薇,李文洁,王卓然,林崇韬. 寡核苷酸YW002对人牙周膜干细胞增殖、周期、凋亡和早期成骨分化的影响[J]. 吉林大学学报(医学版), 2019, 45(2): 273-279. DOI: 10.13481/j.1671-587x.20190211 |
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| 作者姓名： | 毕雪婷 申玉芹 徐晓薇 李文洁 王卓然 林崇韬 |
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| 作者单位： | 吉林大学口腔医院牙周科,吉林长春130021;吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021;吉林大学口腔医院牙周科,吉林长春,130021 |
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| 基金项目： | 国家自然科学基金青年科学基金资助课题（81600879）；吉林省科技厅自然科学基金资助课题（20160101335JC）；吉林省教育厅"十三五"科学技术研究项目资助课题（吉教科合字（2016）485号） |
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| 摘 要： | 目的：探讨免疫刺激型寡核苷酸（ODN） YW002对人牙周膜干细胞（hPDLSCs）增殖、周期、凋亡和成骨分化的影响，阐明ODN YW002对hPDLSCs生物学性能的调控作用。方法：原代培养hPDLSCs至3~5代，将细胞分为空白对照组、免疫抑制型ODN MT01组和ODN YW002组，共培养1、2、3和5d后采用MTT法检测各组hPDLSCs的增殖活性，采用流式细胞术检测各组hPDLSCs的细胞周期和凋亡情况，采用磷酸苯二钠微板法检测各组hPDLSCs中碱性磷酸酶（ALP）活性。结果：与空白对照组比较，ODN YW002组在培养1和3d时hPDLSCs增殖活性升高（P<0.01）；培养5d时hPDLSCs细胞增殖活性升高，但差异无统计学意义（P>0.05）。与ODN MT01组比较，ODN YW002组培养1d时hPDLSCs增殖活性下降（P<0.05）；培养3和5d时细胞增殖活性升高，但差异无统计学意义（P>0.05）。与空白对照组和ODN MT01组比较，ODN YW002组培养1 d时G0/G1期hPDLSCs百分比降低，但差异无统计学意义（P>0.05）；S期hPDLSCs百分比升高，但差异无统计学意义（P>0.05）。与空白对照组比较，ODN YW002组培养1 d时hPDLSCs早期和晚期细胞凋亡率降低（P<0.05）；培养2 d时早期和晚期细胞凋亡率降低，但差异无统计学意义（P>0.05）。与ODN MT01组比较，ODN YW002组培养2d时hPDLSCs早期和晚期细胞凋亡率降低（P<0.05）。与空白对照组比较，培养1、3和5d时ODN YW002组hPDLSCs中ALP活性升高（P<0.01）；与ODN MT01组比较，ODN YW002组培养1和5d时hPDLSCs中ALP活性升高（P<0.05），培养3d时ALP活性降低（P<0.05）。结论：ODN YW002可通过抑制细胞凋亡以促进hPDLSCs增殖，且诱导ALP活性升高，提示其有潜在的促hPDLSCs成骨分化功能。
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| 关 键 词： | 寡核苷酸 牙周膜干细胞 细胞增殖 成骨分化 细胞凋亡 |
| 收稿时间： | 2018-06-15 |
Effects of oligodeoxynucleotide YW002 on proliferation,cell cycle,apoptosis and early osteogenic differentiation of human periodontal ligament stem cells |
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| BI Xueting,SHEN Yuqin,XU Xiaowei,LI Wenjie,WANG Zhuoran,LIN Chongtao. Effects of oligodeoxynucleotide YW002 on proliferation,cell cycle,apoptosis and early osteogenic differentiation of human periodontal ligament stem cells[J]. Journal of Jilin University: Med Ed, 2019, 45(2): 273-279. DOI: 10.13481/j.1671-587x.20190211 |
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| Authors: | BI Xueting SHEN Yuqin XU Xiaowei LI Wenjie WANG Zhuoran LIN Chongtao |
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| Affiliation: | 1. Department of Periodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;2. Key Laboratory of Tooth Development and Bone Remodeling and Regeneration, Jilin Province, Changchun 130021, China |
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| Abstract: | Objective: To investigate the effects of oligodeoxynucleotide(ODN) YW002 on the proliferation, cell cycle, apoptosis and early osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs), and to clarify the regulation effect of ODN YW002 on the biological properties of hPDLSCs.Methods: The HPDLSCs were cultured until the third to the fifth generations. The cells were divided into PBS blank control group, ODN MT01 group and ODN YW002 group and incubated for 1, 2, 3 and 5 d. The proliferation activities of the hPDLSCs in various groups were detected by MTT assay. The cell cycle and apoptosis of the hPDLSCs in various groups were detected by flow cytomety.The alkaline phosphates(ALP) activities of the hPDLSCs in various groups were detected by alkaline phosphatase colorimetric assay.Results: Compared with blank control group, the proliferation activities of hPDLSCs in ODN YW002 group at 1 and 3 d after culture were increased (P<0.01); the proliferation activity was inceased at 5 d after culture, but there was no significant difference(P>0.05). Compared with ODN MT01 group, the proliferation activity of hPDLSCs in ODN YW002 group at 1 d after culture was decreased (P<0.05); the proliferation activities were increased at 3 and 5 d after culture, but there were no significant differences(P>0.05). Compared with blank control and ODN MT01 group, the percentage of hPDLSCs in G0/G1 phase in ODN YW002 group was decreased at 1 d after cultrue,but there was no significant difference(P>0.05); the percentage of hPDLSCs in S phase was increased at 1 d after culture,but there was no significant difference (P>0.05). Compared with blank control group, the early and late apoptotic rates of hPDLSCs in ODN YW002 group were decreased at 1 d after culture (P<0.05);the early and late apoptotic rates were decreased at 2 d after culture, but there were no significant differences(P>0.05).Compared with ODN MT01 group, the early and late apoptotic rates of hPDLSCs in ODN YW002 group were decreased at 2 d after culture (P<0.05). Compared with blank control group, the ALP activities of hPDLSCs in ODN YW002 group were increased at 1, 3 and 5 d after culture(P<0.01).Compared with ODN MT01 group, the ALP activities of hPDLSCs in ODN YW002 group were increased at 1 and 5 d after culture(P<0.05), but the ALP activity was decreased at 3 d after culture (P<0.05).Conclusion: ODN YW002 can promote the proliferation of hPDLSCs by inhibiting the apoptosis, and increase the ALP activity, suggesting that ODN YW002 has the function of promoting the osteogenic differentiation of hPDLSCs. |
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| Keywords: | oligodeoxynucleotide periodontal ligament stem cells cell proliferation osteogenic differentiation apoptosis |
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