| 宫颈癌组织中CDC6、TFF2的表达及其与HPV16/18感染的相关性研究 |
| |
| 引用本文： | 陈丽萍,赵富玺,刘润花. 宫颈癌组织中CDC6、TFF2的表达及其与HPV16/18感染的相关性研究[J]. 癌变．畸变．突变, 2008, 20(6): 459-462. DOI: 10.3969/j.issn.1004-616x.2008.06.011 |
| |
| 作者姓名： | 陈丽萍 赵富玺 刘润花 |
| |
| 作者单位： | 山西大同大学医学院，山西 大同 037008 |
| |
| 摘 要： | 背景与目的： 研究细胞分裂周期蛋白6(CDC6)、三叶因子2(TFF2)在宫颈癌组织中的表达及其与人乳头状瘤病毒(HPV)16/18感染的相关性，以探讨宫颈癌的发生机制。 材料与方法： 选择50例宫颈癌石蜡标本、宫颈上皮内瘤变(CIN)Ⅰ20例、CIN Ⅱ-Ⅲ20例、正常宫颈组织20例，采用免疫组织化学方法检测CDC6、TFF2的表达，同时采用PCR技术检测HPV16/18 DNA，并对 HPV16/18感染情况与CDC6和TFF2表达的相关性进行分析。 结果： 在宫颈癌组织中CDC6的阳性率高于CIN和正常宫颈组织，差异具有统计学意义(P<0.05)，且CDC6阳性表达率与病理分级、淋巴结转移相关，差异具有统计学意义(P均<0.05)。在宫颈癌组织中TFF2的阳性率低于CIN和正常宫颈组织，差异具有统计学意义(P<0.05)，TFF2阳性表达率与年龄分组相关，差异具有统计学意义(P<0.05)，其阳性表达率均与临床分期、病理分级、淋巴转移无相关关系(P均>0.05)；以正常宫颈组织、CIN和宫颈癌为序 HPV16/18 DNA的阳性检出率逐渐升高，各组间的差异具有统计学意义(P<0.05)，但与年龄、临床分期、病理分级、淋巴转移无相关关系(P均>0.05)。在宫颈癌组织中，CDC6与HPV16/18感染呈正相关(rs=0.386, P<0.05)，TFF2与CDC6、HPV16/18感染呈负相关(rs=-0.356,P<0.05;rs=-0.500,P<0.05)。 结论： 宫颈上皮内瘤样病变及宫颈癌组织中CDC6、TFF2表达的改变可能与 HPV16/18感染有关。CDC6的异常表达与宫颈癌的恶变程度有关。
|
| 关 键 词： | CDC6 TFF2 人乳头瘤病毒 宫颈癌 |
| 收稿时间： | 2008-04-15 |
| 修稿时间： | 2008-07-23 |
Relationship between Expression of CDC6 and TFF2 and HPV16/1 8 Infection in Uterine Cervical Cancer |
| |
| CHEN Li-ping,ZHAO Fu-xi,LIU Run-hua. Relationship between Expression of CDC6 and TFF2 and HPV16/1 8 Infection in Uterine Cervical Cancer[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2008, 20(6): 459-462. DOI: 10.3969/j.issn.1004-616x.2008.06.011 |
| |
| Authors: | CHEN Li-ping ZHAO Fu-xi LIU Run-hua |
| |
| Affiliation: | Department of Medcine of Datong University Datong 037008,Shanxi, China |
| |
| Abstract: | BACKGROUND AND AIM: To investigate the relationship between expression of CDC6 and TFF2 and HPV16/18 infection in uterine cervical cancer. MATERIALS AND METHODS: Formalin-fixed, paraffin-embedded samples including 50 cases of squamous cell carcinomas, 20 cervical intraepithelial neoplasia (CIN)Ⅱ-Ⅲ and 20 CINⅠ and 20 samples of normal cervical tissues were studied. Using Polymer DetectionSystem For Immuno-Histological Staining(PV-9000) immunohistochemical technique, we examined the expression of CDC6 and TFF2 in these samples. The infections of HPV type 16, 18 DNA were determined by PCR. RESULTS: There were significant differences for CDC6 among carcinomas, CIN and normal cervices(P<0.05). Expression of CDC6 was correlated with tumor grades and lymph node metastasis (all P<0.05). The positive expression of TFF2 in carcinomas was significantly lower than in normal cervical tissue and CIN (P<0.05). The positive rate of TFF2 was associated with age group (P<0.05)but not with tumor grades, clinical stages and lymph node metastasis(all P>0.05).The positive rate of HPV16/18 was significantly different between cervical carcinomas, CIN and normal tissues (P<0.05) but not between tumor grades, clinical stages, age group and lymph node metastasis (all P>0.05). The expression of CDC6 showed positive correlation with that of HPV16/18 (rs=0.386,P<0.05). HPV16/18 and CDC6 both showed negative correlations with TFF2 (rs=-0.500, P< 0.05;rs=-0.356,P<0.05,respectively). CONCLUSION: In tissues of CIN and cervical carcinomas, changes in CDC6 and TFF2 expression may be associated with HPV16/18 infection. Expressions of CDC6 was associated with the malignant level of uterine cervical cancer. |
| |
| Keywords: | CDC6 TFF2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《癌变．畸变．突变》浏览原始摘要信息 |
|
点击此处可从《癌变．畸变．突变》下载全文 |
|
