| 2010年上海市疑似流行性乙型脑炎患者的病原谱研究 |
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| 引用本文： | 朱兆奎,滕峥,俞雪莲,王嘉瑜,朱奕奕,何懿,周欣,张曦,吴凡. 2010年上海市疑似流行性乙型脑炎患者的病原谱研究[J]. 疾病监测, 2011, 26(11): 848-851. DOI: 10.3784/j.issn.1003-9961.2011.11.004 |
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| 作者姓名： | 朱兆奎 滕峥 俞雪莲 王嘉瑜 朱奕奕 何懿 周欣 张曦 吴凡 |
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| 作者单位： | 1.上海市疾病预防控制中心,上海 200336 |
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| 基金项目： | “十一五”国家科技重大专项(No.2009ZX10004-105,2009ZX10004-211); 上海市卫生局项目(No.2010048)~~ |
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| 摘 要： | 目的 了解上海市疑似流行性乙型脑炎(乙脑)患者的病原谱构成.方法 将2010年5 - 10月收集的145例疑似乙脑患者的93份血清、103份脑脊液标本采用实时荧光PCR(real-time PCR)法和酶联免疫吸附试验(ELIAS)检测常见脑炎病毒核酸和病毒lgM抗体.检测病毒包括乙脑病毒(JEV)、肠道病毒(EV...
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| 关 键 词： | 流行性乙型脑炎 病毒性脑炎 病原谱 实时荧光PCR 酶联免疫吸附试验 |
| 收稿时间： | 2011-05-16 |
Pathogenic spectrum of suspected Japanese encephalitis cases in Shanghai,2010 |
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| ZHU Zhao-kui,TENG Zheng,YU Xue-lian,WANG Jia-yu,ZHU Yi-yi,HE Yi,ZHOU Xin,ZHANG Xi,WU Fan. Pathogenic spectrum of suspected Japanese encephalitis cases in Shanghai,2010[J]. Disease Surveillance, 2011, 26(11): 848-851. DOI: 10.3784/j.issn.1003-9961.2011.11.004 |
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| Authors: | ZHU Zhao-kui TENG Zheng YU Xue-lian WANG Jia-yu ZHU Yi-yi HE Yi ZHOU Xin ZHANG Xi WU Fan |
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| Affiliation: | 1.Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200326, China |
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| Abstract: | Objective To understand the pathogenic spectrum of suspected Japanese encephalitis cases in Shanghai.Methods A total of 93 serum samples and 103 cerebrospinal fluid samples were collected from 145 suspected Japanese encephalitis cases during May-October 2010 to detect viral nucleic acid and IgM antibodies of common encephalitis viruses by using real-time PCR and ELISA.The detected viruses included Japanese encephalitis virus(JEV),enteric virus(EV),cytomegalovirus(CMV),herpes simplex virus(HSV),varicellla-zo... |
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| Keywords: | Japanese encephalitis viral encephalitis pathogenic spectrum real-time PCR enzyme-linked immunosorbent assay |
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