| 柱前衍生化HPLC分析蒲公英多糖的单糖组成 |
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| 引用本文： | 高金波, 孙丽娜. 柱前衍生化HPLC分析蒲公英多糖的单糖组成[J]. 中国现代应用药学, 2010, 27(1): 53-56. |
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| 作者姓名： | 高金波 孙丽娜 |
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| 作者单位： | 1.佳木斯大学药学院，黑龙江 佳木斯 154007 |
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| 基金项目： | 黑龙江省卫生厅科研课题（2007-510） |
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| 摘 要： | 目的 合理优化衍生方法测定蒲公英多糖中单糖的组成及摩尔比。方法 采用1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生，用反相高效液相色谱法进行测定。色谱条件为：ZORBAX-C18柱；流动相：0.1 mol·L-1的磷酸盐缓冲液(pH 6.7)-乙腈(83∶17)；流速：1 mL·min-1；检测波长：245 nm；进样量：20 μL；柱温：室温。结果 蒲公英多糖由D-鼠李糖、葡萄糖、D-半乳糖、D-木糖和D-阿拉伯糖组成，以不同方法提取的各单糖含量的摩尔比为：水提取法，Rha∶Glc∶Gal∶ Ara=0.088∶0.500∶0.190∶0.340；超声提取法，Rha∶Glc∶Gal∶Ara=0.050∶0.350∶0.080∶0.320；酶提取法，Rha∶Glc∶ Gal∶Ara=0.270∶0.630∶0.330∶0.460；超声协提取同酶法，Rha∶Glc∶Gal∶Ara =0.078∶0.550∶0.130∶0.170。结论 改进后的衍生化方法和等度洗脱的色谱方法具有操作简单、快速、重复性好等特点，可用于蒲公英多糖中单糖组成和摩尔比的测定。
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| 关 键 词： | 柱前衍生化 高效液相色谱法 单糖 1-苯基-3-甲基-5-吡唑啉酮 |
Analysis on Monosaccharide Composition of Taraxacum Polysaccharides by Pre-column Derivatization HPLC |
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| GAO Jinbo, SUN Lina. Analysis on Monosaccharide Composition of Taraxacum Polysaccharides by Pre-column Derivatization HPLC[J]. Chinese Journal of Modern Applied Pharmacy, 2010, 27(1): 53-56. |
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| Authors: | GAO Jinbo SUN Lina |
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| Affiliation: | 1.College of Chemistry and Pharmacy of Jiamusi University, Jiamusi 154007, China |
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| Abstract: | OBJECTIVE To optimize the derivative method reasonably and detect monosaccharide components and molar ratio of taraxacum polysaccharides.METHODS Pre-column derivative reagent was 1-phenyl-3-methyl-5-pyrazolone（PMP）,monosaccharide composition and molar ratio of taraxacum polysaccharides were detected by reversed-phase high performance liquid chromatography（RP-HPLC）.Chromatographic conditions：ZORBAX-C18 column;mobile phase：0.1 mol·L^-1 phosphate buffer solution（pH 6.7）-acetonitrile（83 ：17）;velocity：1 mL·min^-1;detection wavelength：245 nm;injection volume：20 μL;column temperature：room temperature.RESULTS Taraxacum polysaccharides was composed of D-rhamnose,glucose,D-galactose D-xylose,D-Arabinose.The molar ratio of taraxacum polysaccharides which was extracted by different means was as forrows：water extraction method,Rha：Glc：Gal：Ara=0.088 ：0.500 ：0.190 ：0.340;ultrasonic extraction method,Rha：G lc：G al：A ra=0.050：0.350：0.080：0.320;cellulose extraction method,Rha：G lc：G al：A ra=0.270：0.630：0.330：0.460;synergistic ultrasonic extraction and cellulose extraction,Rha：Glc：Gal：Ara=0.078 ：0.550 ：0.130 ：0.170.CONCLUSION The improved derivative method and the chromatographic conditions of isocratic elution were simple,rapid and with a good reproducibility,and can be used to detect monosaccharide components and molar ratio of taraxacum polysaccharides. |
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| Keywords: | pre-column derivatization HPLC monosaccharide 1-phenyl-3-methyl-5-pyrazolone |
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