Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment |
| |
| Authors: | Rolink A G Winkler T Melchers F Andersson J |
| |
| Affiliation: | Basel Institute for Immunology, CH-4005 Basel, Switzerland. rolink@bii.ch |
| |
| Abstract: | The capacity of precursor B (pre-B) I cells from fetal liver and bone marrow to proliferate and differentiate into surface immunoglobulin-positive immature B cells in vitro was analyzed. Both fetal liver- and bone marrow-derived progenitors do so in a pre-B cell receptor (pre-BCR)-dependent manner in tissue culture medium alone, without addition of other cells or cytokines. Approximately 20% of the initial pre-B I cells enter more than one division. Analyses at the single-cell level show that approximately 15% divide two to five times. Coculture of pre-B I cells with stromal cells did not enhance proliferation or differentiation, whereas the presence of interleukin 7, especially in combination with stromal cells, resulted mainly in the expansion of pre-B I cells and prevented their further differentiation. Thus, the environment of fetal liver or bone marrow is not required for the pre-BCR to exert its function, which is to select and expand cells that have undergone an inframe V(H)-D(H)J(H) rearrangement that produces a pre-BCR-compatible muH chain. It appears unlikely that a ligand for the pre-BCR drives this pre-B cell proliferation. |
| |
| Keywords: | B cell development precursor B cell receptor λ5 c-kit bcl-2 |
| 本文献已被 PubMed 等数据库收录！ |
|
