| 脱氢表雄酮对大鼠实验性自身免疫性神经炎的影响 |
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| 引用本文： | 谭晓冬,段瑞生,时昌文,孙若鹏. 脱氢表雄酮对大鼠实验性自身免疫性神经炎的影响[J]. 山东大学学报(医学版), 2008, 46(9): 837-841 |
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| 作者姓名： | 谭晓冬 段瑞生 时昌文 孙若鹏 |
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| 作者单位： | 山东省千佛山医院儿科,济南,250014;山东大学齐鲁医院儿科,济南,250812 |
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| 摘 要： | 目的探讨脱氢表雄酮（DHEA）对大鼠实验性自身免疫性神经炎（EAN）发病的影响及其机制。方法随机将36只Lewis大鼠分为DHEA 0.5mg治疗组、2mg治疗组和对照组。治疗组于注射牛周围神经髓磷脂（BPM）抗原乳剂后第5天开始每日皮下注射DHEA，对照组皮下注射相同体积的DHEA溶媒。观察各组发病时间及临床评分,检查疾病高峰期坐骨神经组织病理及干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）阳性反应细胞，检测引流淋巴结和脾脏单个核细胞增殖反应，检测培养上清TNF-α、IFN-γ、白介素-10（IL-10）含量。 结果两种剂量DHEA治疗组均能延迟EAN发病时间(P均<0.05)，减少坐骨神经炎性细胞浸润数目(P均<0.05)和IFN-γ、TNF-α阳性反应细胞数目(P均<0.05)，抑制BPM刺激T淋巴细胞增殖(P均<0.05)，降低培养上清中IFN-γ、TNF-α水平(P均<0.05)。2?mg治疗组疾病高峰期临床评分明显降低于对照组(P<0.05)。各组间 IL-10水平未显示明显差异（P>0.05）。结论DHEA可能通过抑制自身反应性T淋巴细胞增殖和促炎性细胞因子过度表达减轻EAN病情，2mg治疗组具有更明显的免疫治疗效果。
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| 关 键 词： | 神经炎 实验性变应性 脱氢(表)雄甾酮 细胞因子类 大鼠 近交Lew |
| 收稿时间： | 2008-04-17 |
Effects of dehydroepiandrosterone on experimental autoimmune neuritis in Lewis rats |
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| TAN Xiao-dong,DUAN Rui-sheng,SHI Chang-wen,SUN Ruo-peng. Effects of dehydroepiandrosterone on experimental autoimmune neuritis in Lewis rats[J]. Journal of Shandong University:Health Sciences, 2008, 46(9): 837-841 |
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| Authors: | TAN Xiao-dong DUAN Rui-sheng SHI Chang-wen SUN Ruo-peng |
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| Affiliation: | 1. Department of Pediatrics， Qianfoshan Hospital Affiliated to Shandong University， Jinan 250014， China；2. Department of Pediatrics， Qilu Hospital of Shandong University， Jinan 250012， China |
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| Abstract: | To explore the effects of dehydroepiandrosterone (DHEA) on the development of experimental autoimmune neuritis (EAN) and to explore its mechanism. Methods Thirty-six Lewis rats were randomly divided into the DHEA 0.5mg treatment group, the DHEA 2mg treatment group and the control group (n=12). The treatment groups were subcutaneously injected every day with DHEA and the control group was subcutaneously injected with the same volume of DHEA dissolvent from day 5 post-immunization (p.i.) to day 28 p.i. with bovine peripheral myelin（BPM）in Freund′s complete adjuvant (CFA). The effects were assessed in terms of appearance of clinical signs, clinical score, histopathology and IFN-γ and TNF-α positive cells in sciatic nerve sections, and T-cell proliferation and inflammatory cytokines (IFN-γ,TNF-α and IL-10) synthesis by draining lymph nodes and spleen cells. ResultsRats receiving DHEA in the two different therapeutic regimens displayed a significant delay in onset (P<0.05 and P<0.05), a decreased inflammatory cell infiltration into the PNS (P<0.05,P<0.05) and decreased numbers of IFN-γ and TNF-α expression cells in the PNS(P<0.05 and P<0.05 ), and reduced BPM stimulated T cell proliferation(P<0.05 and P<0.05) and IFN-γ and TNF-α secretion in the draining lymph node and spleen (P<0.05, P<0.05) compared to the control group. Only the DHEA 2mg treatment group had the significant differences of supernatant IL-10 were found among the different treatment and control groups（P>0.05）.ConclusionAdministration of exogenous DHEA ameliorates the severity of EAN, presumably by suppressing the proliferation of auto-reactive T-cells and moderating the over-expression of pro-inflammatory cytokines. DHEA 2mg therapy is more effective than DHEA 0.5mg therapy. |
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| Keywords: | Neuritis experimental allergic Dehydroepiandrosterone Cytokines Rats inbred Lew |
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