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| 以重组蛋白为抗原的ELISA法检测旋毛虫抗体 | |
| 引用本文： | 牛廷献,潘菲菲,刘明远,卢强,付宝权,Pascal Boireau. 以重组蛋白为抗原的ELISA法检测旋毛虫抗体[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(3): 143-145 |
| 作者姓名： | 牛廷献 潘菲菲 刘明远 卢强 付宝权 Pascal Boireau |
| 作者单位： | 1. 兰州军区总医院动物实验科,兰州,730050 2. 兰州军区五里铺干休所,兰州,730050 3. 解放军军需大学军事兽医系,长春,130062 4. 法国食品卫生安全局,法国,巴黎,94703 |
| 基金项目： | 国家自然科学基金(NSFC-30170709)，中法先进计划项目(9612)~~ |
| 摘 要： | 目的 寻求特异性强、敏感性高的旋毛虫病诊断抗原。 方法 以旋毛虫新生幼虫期特异性T668基因在E.coli高效表达的重组蛋白为抗原，分别以兔、猪和健康者血清及旋毛虫病阳性血清为一抗，以辣根过氧化物酶（HRP）标记的山羊抗兔IgG、山羊抗猪IgG和山羊抗人IgG为二抗，建立检测旋毛虫抗体的间接ELISA方法，并以旋毛虫肌幼虫排泄?鄄分泌（ES）抗原作为检测对照。 结果 以T668重组蛋白为抗原，对兔、猪和人旋毛虫病血清进行检测，阳性检出率为100 ％，且敏感性高（0.016 μg / 孔），与ES抗原检测结果完全一致。 结论 T668重组抗原有望替代ES抗原检测旋毛虫抗体。 |
| 关 键 词： | 旋毛虫 重组蛋白 新生幼虫期特异性基因 间接ELISA |
| 文章编号： | 10000-7423(2005)-03-0143-03 |
| 修稿时间： | 2004-09-16 |
Detection of Anti-Trichinella spiralis Antibody by Indirect ELISA Using Recombinant Protein as Antigen |
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| NIU Ting-xian,PAN Fei-Fei,LIU Ming-yuan,LU Qiang,FU BAO-QUAN,Pascal Boireau. Detection of Anti-Trichinella spiralis Antibody by Indirect ELISA Using Recombinant Protein as Antigen[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 23(3): 143-145 | |
| Authors: | NIU Ting-xian PAN Fei-Fei LIU Ming-yuan LU Qiang FU BAO-QUAN Pascal Boireau |
| Affiliation: | Lanzhou General Hospital of PLA, Lanzhou 730050, China. |
| Abstract: | OBJECTIVE: To study the specificity, sensitivity of diagnostic antigen for Trichinella spiralis (T.s). METHODS: T668 recombinant protein from highly efficient expression of newborn larvae stage-specific gene of T.s in E. coli as diagnostic antigen. By using negative and positive sera from rabbit, pig, human as the first antibody, and goat-anti-rabbit IgG, goat-anti-pig IgG, goat-anti-human IgG labeled with HRP as the secondary antibody, indirect ELISA method was established for detecting anti-T.s antibody, while excretory-secretory (ES) antigen of T.s muscle larvae was used as control. RESULTS: Sera from rabbit, pig and human were detected by T668 recombinant protein as antigen, which showed a positive rate of 100% with 0.016 microg/well. There was no difference on the results between the T668 recombinant antigen and the ES antigen for diagnosing T.s-infection. CONCLUSION: The T668 recombinant antigen is promising in substituting ES antigen in the detection of anti-T.s antibody. |
| Keywords: | Trichinella spiralis Recombinant protein Newborn larvae (NBL) stage-specific gene Indirect ELISA |
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