| 仙人掌多糖对大鼠局灶性脑缺血的神经保护作用 |
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| 引用本文： | 唐焜,谢小慧,陈志达,陈扬,郭莲军. 仙人掌多糖对大鼠局灶性脑缺血的神经保护作用[J]. 医药导报, 2012, 31(9): 1109-1112. DOI: 10.3870/yydb.2012.09.001 |
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| 作者姓名： | 唐焜 谢小慧 陈志达 陈扬 郭莲军 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院第二临床学院,武汉430030;华中科技大学同济医学院基础医学院药理学系,武汉430030
2. 华中科技大学同济医学院基础医学院药理学系,武汉,430030 |
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| 基金项目： | 中央高校基本科研业务费专项资金资助项目(2011TS073);华中科技大学研究生科技创新基金资助项目;华中科技大学2011年第二批大学生科技创新基金资助项目;华中科技大学2012年第一批大学生创业创新基金资助项目(104872012169) |
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| 摘 要： | 摘要目的观察仙人掌多糖对大脑中动脉栓塞（MCAO）诱导的大鼠缺血 再灌注损伤的神经保护作用。方法雄性SD大鼠随机分为假手术组、缺血组、0.9%氯化钠溶液组、仙人掌多糖治疗组（200 mg·kg－1·d －1，ip）。采用MCAO法制作大鼠缺血 再灌注损伤模型。分别于大鼠缺血2 h再灌注3，6，8 h后进行神经行为学评分；8 h后2，3，5 三苯基氯化四氮唑（TTC）染色测定脑梗死体积；缺血2 h再灌注22 h后脑组织切片苏木精 伊红（HE）染色显微镜下形态学观察。结果与0.9%氯化钠溶液组相比，再灌注8 h后仙人掌多糖治疗组神经行为学评分平均下降（2.35±0.47）分（P＜0.05），梗死灶体积减少（P＜0.05）；大鼠皮质及海马组织神经细胞丢失、神经胶质增生、核固缩、核深染等形态学均有明显改善（P＜0.05）。结论仙人掌多糖可以缓解大鼠大脑中动脉栓塞症状，具有一定的神经保护作用。
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| 关 键 词： | 仙人掌多糖 大脑中动脉栓塞 缺血-再灌注 神经保护 |
Neuroprotective Effect of Cactus Polysaccharides on Focal Cerebral Ischemia in Rats |
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| TANG Kun , XIE Xiao-hui , CHEN Zhi-da , CHEN Yang , GUO Lian-jun. Neuroprotective Effect of Cactus Polysaccharides on Focal Cerebral Ischemia in Rats[J]. Herald of Medicine, 2012, 31(9): 1109-1112. DOI: 10.3870/yydb.2012.09.001 |
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| Authors: | TANG Kun XIE Xiao-hui CHEN Zhi-da CHEN Yang GUO Lian-jun |
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| Affiliation: | 1.Second Clinical Department,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;2.Department of Pharmacology,School of Basic Medical Sciences,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China) |
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| Abstract: | Objective To study the neuroprotective effect of cactus polysaccharides(CP) on ischemia/reperfusion(I/R) injury induced by the middle cerebral artery occlusion(MCAO) in rats.Methods The male SD rats were randomly divided into sham-operated,MCAO/R model group,NS-treated group,CP-treated group(200 mg·kg-1·d-1,ip).The MCAO/R model was made by MCAO method.The neurological deficits were detected at 3 h,6 h and 8 h after MCAO for 2 h and reperfusion;The cerebral infarct volume was detected by TTC staining 8 h later,the brain tissue sections of MCAO for 2 h and reperfusion for 22 h were stained by hematoxylin-eosin(H&E) for the morphological observation under microscope.Results Compared to the NS-treated group,the neurological deficit scores of CP-treated group after reperfusion for 8 h was decreased by(2.35±0.47)(P<0.05),in company with the loss of infarct volume(P<0.05).The rat cortical and hippocampal neuronal cell loss,gliosis,nuclear condensation and nuclear deep dyeing were significantly alleviated(P<0.05).Conclusion The CP can attenuate of arterial embolism in rats and ameliorate cerebral ischemia,which possessing a neuroprotective effect. |
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| Keywords: | Cactus polysaccharides(CP) Middle cerebral artery occlusion(MCAO) Ischemia/reperfusion(I/R)injury Neuroprotection |
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