| 呼吸道合胞病毒毛细支气管炎患儿血白细胞5-脂氧酶及其激活蛋白mRNA水平检测 |
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| 引用本文： | 黄柳一,岳智慧,孙良忠,王青梅. 呼吸道合胞病毒毛细支气管炎患儿血白细胞5-脂氧酶及其激活蛋白mRNA水平检测[J]. 中山大学学报(医学科学版), 2011, 32(3): 370-373 |
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| 作者姓名： | 黄柳一 岳智慧 孙良忠 王青梅 |
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| 作者单位： | 1. 中山大学附属第一医院儿科,广东 广州 510080;2.广州市儿童医院儿科,广东 广州 510000 |
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| 摘 要： | 【目的】 观测呼吸道合胞病毒(RSV)毛细支气管炎患儿外周血白细胞5脂氧酶(5-LO)mRNA及5脂氧酶激活蛋白(FLAP)mRNA水平及其与临床严重程度关系。 【方法】 17例鼻咽分泌物RSV检测阳性的毛细支气管炎患儿为病例组;同期入院的非感染性外科疾病术前患儿10例为对照组,两组年龄分布及性别构成均无显著性差异。采用荧光定量PCR检测两组患儿外周血白细胞5-LO及FLAP mRNA水平,比较两组患儿血白细胞5-LO及FLAP mRNA水平的差异并分析其与临床严重程度的关系。【结果】 病例组患儿外周血白细胞5-LO(0.2364 ± 0.0332)和FLAP (0.4106 ± 0.0575) mRNA水平均较对照组(分别为0.0018 ± 0.0013和0.0044 ± 0.0030)明显升高,P值均 < 0.01。病例组患儿外周血白细胞5-LO 和FLAP mRNA水平与临床严重程度评分均呈正相关(r分别为0.745, 0.594,P值均 < 0.05)。【结论】 RSV毛细支气管炎患儿外周血白细胞5-LO mRNA及FLAP mRNA水平升高并与临床严重程度呈正相关。
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| 关 键 词： | 呼吸道合胞病毒 毛细支气管炎 5脂氧酶 5脂氧酶激活蛋白 |
| 收稿时间： | 2010-12-25; |
Expression of 5-Lipoxygenase mRNA and 5-Lipoxygenase-activating Protein mRNA in the Peripheral Blood White Blood Cells in Respiratory Syncytial Virus Bronchiolitis Children |
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| HUANG Liu-yi,YUE Zhi-hui,SUN Liang-zhong,WANG Qing-mei. Expression of 5-Lipoxygenase mRNA and 5-Lipoxygenase-activating Protein mRNA in the Peripheral Blood White Blood Cells in Respiratory Syncytial Virus Bronchiolitis Children[J]. Journal of Sun Yatsen University(Medical Sciences), 2011, 32(3): 370-373 |
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| Authors: | HUANG Liu-yi YUE Zhi-hui SUN Liang-zhong WANG Qing-mei |
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| Affiliation: | 1.Department of Pediatrics, The First Affiliated Hospital, Sun Yat-Sen University,Guangzhou 510080,China, 2. Guangzhou Children Hosipital,Guangzhou 510000, China |
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| Abstract: | [Objective] To observe 5-lipoxygenase(5-L0) mRNA level and 5-lipoxygenase-Activating protein(FLAP) mRNA level of the peripheral blood white blood cells(WBC) and their correlation to the clinical manifestation in RSV bronchiolitis children.[Methods] Seventeen children suffered from bronchiolitis whose nasopharyngeal secretion positive for RSV-Ag were put into patient group.10 preoperative patients with matched age,gender proportion and no evidence of infection were chosen as control group.5-LO mRNA level and... |
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| Keywords: | respiratory syncytial virus bronchiolitis 5-lipoxygenase 5-lipoxygenase-activating protein |
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