| 不同剂量链脲佐菌素诱发长爪沙鼠高血糖模型的初步观察 |
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| 引用本文： | 刘锋华,郭红刚,楼琦,戴方伟,卢领群,萨晓婴,周光兴. 不同剂量链脲佐菌素诱发长爪沙鼠高血糖模型的初步观察[J]. 实验动物与比较医学, 2010, 30(5): 335-339. DOI: 10.3969/j.issn.1674-5817.2010.05.005 |
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| 作者姓名： | 刘锋华 郭红刚 楼琦 戴方伟 卢领群 萨晓婴 周光兴 |
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| 作者单位： | 1. 复旦大学实验动物科学部,上海,200032
2. 浙江省医学科学院实验动物中心;浙江省实验动物与安全性研究重点实验室,杭州310013 |
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| 基金项目： | 浙江省科技厅科技项目 |
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| 摘 要： | 目的 探讨链脲佐菌素（STZ）诱发长爪沙鼠高血糖模型的建立及其合适药物剂量. 方法 40只成年长爪沙鼠,按照正常对照组、150 mg/kg,200 mg/Kg、250 mg/Kg、400 mg/kg和600 mg/kg的剂量组,随机分为A、B、C、D、E、F6个组.按照设定的时间点监测60d内各组沙鼠的血糖、体重及24 h尿量变化. 结果 C、D组沙鼠注射STZ 24 h后,血糖值均数升高16.65 mmol/L以上,72 h后临时性降低,但仍高于16.65 mmol/L,之后的4 d血糖值波动性升高至25 mmol/L左右,7 d后的血糖值均数持续稳定在该水平,注射STZ 7 d后动物成模率100%,且在实验周期2个月内动物死亡率较低,无自发性缓解率,出现典型的＂三多一少＂体征（多饮、多食、多尿、消瘦）.B组沙鼠注射STZ 7 d后,动物成模率62.5%（5/8）,死亡率O,两个月内高血糖维持率25%（2/8）.E、F组沙鼠注射STZ 7d后,死亡率分别为83.30%（5/6）、100%. 结论 一次性大剂量注射链脲佐菌素能诱发长爪沙鼠持续性血糖升高,并可建立稳定的长爪沙鼠高血糖模型,沙鼠高血糖标准为空腹或非空腹血糖≥16.65 mmol/L,STZ的合适剂量为200～250 mg/kg.
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| 关 键 词： | 链脲佐菌素 长爪沙鼠 高血糖模型 剂量 |
Primary Observation on Hyperglycemic Models in Mongolian Gerbils Induced by Different Dosage of Streptozotocin |
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| LIU Feng-hu,GUO Hong-gang,LOU Qi,DAI Fang-wei,LU Ling-qun,SA Xiao-ying,ZHOU Guang-xing. Primary Observation on Hyperglycemic Models in Mongolian Gerbils Induced by Different Dosage of Streptozotocin[J]. Laboratory Animal and Comparative Medicine, 2010, 30(5): 335-339. DOI: 10.3969/j.issn.1674-5817.2010.05.005 |
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| Authors: | LIU Feng-hu GUO Hong-gang LOU Qi DAI Fang-wei LU Ling-qun SA Xiao-ying ZHOU Guang-xing |
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| Affiliation: | 1. Department of Laboratory Animal Sciences, Fudan University, Shanghai 200032, China; 2. Experimental Animal Research Center of Zhejiang Academy of Medical Sciences, Zhejiang Key Laboratory of Experimental Animal and Safety Research,Hangzhou 30013, China) |
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| Abstract: | Objective To explore the proper dosage of streptozotocin （STZ） in the hyperglycemic models in the Mongolian gerbils induced by STZ. Methods The forty Mongolian gerbils were divided randomly into normal control group： A group intraperitoneally （i.p.） injected with acid citrate buffer solution and hyperglycemic groups： B, C, D, E, F group i.p. injected with STZ at the dose of 150, 200, 250, 400, 600 mg/kg body weight, respectively. The levels of blood glucose, the body weights and 24 h urine volume in two months were tested accordingly. Result Twenty-four hours after STZ injection, blood glucose concentration of the gerbils in C and D group are more than 16.65 mmol/L and shortly decreased at no less than 16.65 mmol/L since 72 hours after STZ injection, in the following 4 days, blood glucose rose up to 25 mmol/L, the nonfasting blood glucose level became relatively stable since 7 days after STZ injection in Mongolian gerbils, which were kept under observation for 2 months. These hyperglycemic Mongolian gerbils in C group and D group which had higher achievement ratio, lower mortality and lower rate of spontaneous recovery had distict diabetic symptoms （Polyuria, polydipsia, hyperphagia and weight loss） with hyperglycemia during the experiment period. Seven days after STZ injection, the achievement ratio of gerbils in B group was 62.5%（5/8） and the maintenance rate of hyperglycemia during the experiment period was 25%（2/8）. The mortality of gerbils in E and F group were 83.30%（5/6） and 100% since 7 days after STZ injection. Conclusion The stable hyperglycemic models in the Mongolian gerbils can be induced by large dose of streptozotocin once,the standard of hyperglycemic model in the Mongolian gerbils is that either the fasting blood glucose or nonfasting blood glucose is above 16.65 mmol/L, the proper dosage of streptozotocin （STZ） may be between 200 mg/kg and 250 mg/kg. |
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| Keywords: | Streptozotocin Mongolian gerbils Hyperglycemic models Dosage |
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