Nucleosome core particles suppress the thermal untwisting of core DNA and adjacent linker DNA. |
| |
| Authors: | R H Morse and C R Cantor |
| |
| Abstract: | Covalently closed circular DNA is known to undergo a temperature-dependent change in helical twist. We have reconstituted nucleosome core particles onto closed circular DNA and measured the thermal untwisting of the DNA as a function of nucleosome density. The results demonstrate that the DNA associated with the nucleosome core particle does not alter its twist when the temperature is varied between 4 degrees C and 37 degrees C, and that the length of DNA prevented from thermal untwisting includes the linker as well as the core DNA. |
| |
| Keywords: | |
|
|
