HOST SPECIFICITY OF DNA PRODUCED BY Escherichia coli, XI. In vitro MODIFICATION OF PHAGE FD REPLICATIVE FORM |
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| Authors: | Urs Kü hnlein, Stuart Linn, Werner Arber |
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| Affiliation: | Urs Kühnlein, Stuart Linn, and Werner Arber |
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| Abstract: | An enzymatic activity, having the properties expected of a B-specific host-controlled modification enzyme, has been purified from an extract of Escherichia coli strain B. This activity renders the unmodified replicative form of phage fd resistant to B-specific restriction and is only present in strains carrying intact genes for type B modification. In phosphate buffer, the enzyme acts optimally at pH 6 and is dependent upon a single cofactor, S-adenosylmethionine. |
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