Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses |
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| Authors: | Kalpoe J S Templeton K E Horrevorts A M Endtz H P Kuijper E J Bernards A T Klaassen C H W |
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| Affiliation: | Department of Medical Microbiology, E4-P, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands. J.S.Kalpoe@lumc.nl |
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| Abstract: | Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates. |
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