| 重组人酸性成纤维细胞生长因子皮肤用药的长期毒性研究 |
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| 引用本文： | 重组人酸性成纤维细胞生长因子皮肤用药的长期毒性研究[J]. 中国药科大学学报, 2003, (1): 48-52. |
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| 作者姓名： | 许华 李校堃 郑青 杨晓明 黄亚东 赵文 姚成灿 吴晓萍 黄芝瑛 |
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| 作者单位： | 1. 暨南大学药学院医药生物技术研究开发中心,广州,510632
2. 广州市医药工业研究所,广州,510240 |
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| 基金项目： | 国家高技术研究发展计划 (十五 863)项目 (编号 2 0 0 1AA2 15 131)~~ |
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| 摘 要： | 目的：研究重组人酸性成纤维细胞生长因子（rhaFGF)皮肤用药的长期毒性。方法：对新西兰兔破损皮肤连续给药28d，剂量分别为3600，1800，900U／cm^2,观察rhaFGF用药期间和停药后对动物血液学、血液生化学、组织学等方面的影响。结果：rhaFGF的无毒剂量为900U／cm^2;rhaFGF1800U/cm^2用药可见个别动物出现淋巴结、脾白髓、圆小囊固有层及胸腺滤泡增生；3600U／cm^2用药可见半以上动物淋巴结出现淋巴等器官滤泡增生，偶见大脑血管周围出现少量淋巴细胞浸润，个别动物睾丸生精小管精子细胞数量增加。停药2周，上述病理改变减轻。未观察到rhaFGF的延迟性毒性反应。结论：rhaFGF在有效剂量及大于有效15倍的剂量下皮肤外用是安全的。
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| 关 键 词： | 重组人酸性成纤维细胞生长因子 皮肤用药 长期毒性 |
| 文章编号： | 1000-5048(2003)01-0046-05 |
| 修稿时间： | 2002-07-15 |
Study on Long-term Toxicity of Recombinant Human Acidic Fibroblast Growth Factor in Rabbits |
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| Study on Long-term Toxicity of Recombinant Human Acidic Fibroblast Growth Factor in Rabbits[J]. Journal of China Pharmaceutical University, 2003, (1): 48-52. |
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| Abstract: | AIM:To investigate the long tern toxicity of recombinant human acidic fibroblast growth factor (rhaFGF) in rabbits.METHOD:The long term toxicity was tested in rabbits by daubed rhaFGF to scathe skin at the dose of 3600,1800,900 U/cm 2 respectively, once daily for 28 successive days. The hematological, blood biochemical, pathological examination and so on were conducted at 28 days after treatment or at 14 days after stopping administration. RESULT:No abnormal hematological, blood biochemical, pathological index were found in the group of 900 U/ cm 2. The hyperplasia of lymph gland, splenic white pulp, lamina propria of roundlet vesicular organ,and thymus follicles could be observed in a few of rabbits in the group of 1800 U/cm 2. In the group of 3600 U/cm 2, the hyperplasia above mentioned was observed in moiety rabbits, lymphocyte infiltration all round the brain blood could be seen occasionally, and the count of seminiferous tubule testis increased in a modicum of rabbits. The pathologic changes above mentioned were lessenod after stopping treatment of two weeks. The postpone toxic reaction of rhaFGF was not observed. CONCLUSION:rhaFGF used by skin administration at the dose of efficacy and at the most 15 fold of effective dose is safe. |
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| Keywords: | rhaFGF Long term toxicity Skin administration |
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