Identification of mycobacteria by high-performance liquid chromatography. |
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| Authors: | W R Butler K C Jost Jr J O Kilburn |
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| Affiliation: | Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333. |
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| Abstract: | Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described. |
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