Are many Z-DNA binding proteins actually phospholipid-binding proteins? |
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| Authors: | P Krishna B P Kennedy D M Waisman J H van de Sande J D McGhee |
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| Affiliation: | Department of Medical Biochemistry, University of Calgary, AB, Canada. |
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| Abstract: | We used a Z-DNA affinity column to isolate a collection of Z-DNA binding proteins from a high salt extract of Escherichia coli. We identified one of the major Z-DNA binding proteins of this fraction, not as a protein involved in gene regulation or genetic recombination, but rather as an outer membrane porin protein. We then showed that several other known phospholipid-binding proteins (bovine lung annexins and human serum lipoproteins) also bind much more tightly to Z-DNA than to B-DNA. In all cases, this Z-DNA binding was strongly blocked by competition with acidic phospholipids, such as cardiolipin. Our results raise the question whether many of the Z-DNA binding proteins previously isolated are actually phospholipid-binding proteins. |
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