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| 黑暗链霉菌spu46产生的卡那霉素B的分离提取 | |
| 引用本文： | 于游,王曼丽,倪现朴,夏焕章. 黑暗链霉菌spu46产生的卡那霉素B的分离提取[J]. 沈阳药科大学学报, 2011, 28(4): 316-319 |
| 作者姓名： | 于游 王曼丽 倪现朴 夏焕章 |
| 作者单位： | 1.中国医科大学 附属第四医院，辽宁 沈阳 110032；2.中国石油新疆油田公司明园职工医院，新疆 乌鲁木齐 830000；3.沈阳药科大学 生命科学与生物制药学院，辽宁 沈阳 110016 |
| 摘 要： | 目的建立分离提取高纯度卡那霉素B的最佳工艺条件。方法以基因工程黑暗链霉菌spu46发酵产物为原料,通过对树脂吸附性能的研究,筛选吸附容量最大的弱酸性树脂;考察上样液的pH、浓度、流速等因素对吸附效果的影响;考察不同洗脱条件对分离提纯效果的影响。结果弱酸性阳离子树脂CD-180在pH 7.5、流速0.85 mL.m in-1的条件下,吸附量达到最高。0.15 mol.L-1的氨水在1.00 mL.m in-1,的流速下,解析率稳定,可达到85%以上。结论弱酸性阳离子树脂CD-180对卡那霉素B的分离纯化效果最佳。 |
| 关 键 词： | 卡那霉素B 分离 纯化 CD180树脂 |
| 收稿时间： | 2010-04-21 |
Extraction process of kanamycin B from Streptomyces tenebrarius spu46 |
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| YU You,WANG Man-li,NI Xian-pu,XAI Huan-zhang. Extraction process of kanamycin B from Streptomyces tenebrarius spu46[J]. Journal of Shenyang Pharmaceutical University, 2011, 28(4): 316-319 | |
| Authors: | YU You WANG Man-li NI Xian-pu XAI Huan-zhang |
| Affiliation: | 1.The fouth Afliated Hospital, China Medical University, Shenyang 110032, China; 2. Mingyuan Staff-Worker Hospital of Xinjiang Oil Field Company, Ueumqi 830000, China; 3. Shool of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China |
| Abstract: | Objective To determine the best extraction process of kanamycin B. Methods Based on the fermentation broth of Streptomyces tenebrarius spu46 producing kanamycin B, static adsorption and dynamic adsorption were studied to choose resin with the best adsorption capability. Effect of pH value, concentration and flow rate on performance of absorption was investigated using the chosen resin. Effect of different desorption parameter on extraction was also researched. Results Weakly acidic cationic resin had the best performance of absorption with pH7.5 and flow rate of 0.85 mL•min-1. Desorption rate could reach 85% when concentration of ammonia water was 0.15 mol•L-1, with flow rate of 1.00 mL•min-1. Conclusion Weakly acidic cationic resin CD180 has the best capability on the extraction of kanamycin B. |
| Keywords: | kanamycin B extraction purification CD180 resin |
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