首页 | 本学科首页   官方微博 | 高级检索  
     


Instability of Trinucleotidic Repeats During Chromatin Remodeling in Spermatids
Authors:Olivier Simard  Marie‐Chantal Grégoire  Mélina Arguin  Marc‐André Brazeau  Frédéric Leduc  Isabelle Marois  Martin V. Richter  Guylain Boissonneault
Affiliation:1. Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, Canada;2. Department of Medicine, Université de Sherbrooke, Sherbrooke, Quebec, Canada
Abstract:Transient DNA breaks and evidence of DNA damage response have recently been reported during the chromatin remodeling process in haploid spermatids, creating a potential window of enhanced genetic instability. We used flow cytometry to achieve separation of differentiating spermatids into four highly purified populations using transgenic mice harboring 160 CAG repeats within exon 1 of the human Huntington disease gene (HTT). Trinucleotic repeat expansion was found to occur immediately following the chromatin remodeling steps, confirming the genetic instability of the process and pointing to the origin of paternal anticipation observed in some trinucleotidic repeats diseases.
Keywords:double‐strand break  genetic instability  Huntington disease  spermatid chromatin remodeling  trinucleotide expansion  Huntington  HTT
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号