Purification,characterization, in vitro anti-hepatic fibrosis activity of bioactive peptides derived from Carapax Trionycis hydrolysates |
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| Authors: | Chunling Hu Xiaozhi Peng Yinping Tang Yanwen Liu Zuliang Hu Jianrong Gao |
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| Abstract: | Anti-hepatic fibrosis peptide from Carapax Trionycis was purified, characterized, and inhibitory effect was assessed. Carapax Trionycis extract peptide hydrolysates (CTEPHs) were separated by ultrafiltration, Sephadex G-15 gel chromatography and RP-HPLC. One novel anti-hepatic fibrosis peptide (CTEPH-1: Asn-Pro-Asn-Pro-Thr) was obtained and identified. MTS assay and enzyme-linked immunosorbent assay were applied to evaluate the anti-fibrotic effect of CTEPH-1 on activated hepatic stellate cells (HSCs) in vitro. CTEPH-1 efficiently inhibited activation and proliferation of cultured HSC-T6 cells via lowering the contents of collagen and TIMP-1 except for matrix metalloproteinase-1 (MMP-1). The purified peptide might be beneficial as functional food or potential drug for treatment of liver fibrogenesis. |
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| Keywords: | Carapax Trionycis extract peptide hydrolysates Extracellular matrix Hepatic stellate cells |
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