| 两种红豆杉属植物细胞培养技术的建立和优化 |
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| 引用本文： | 两种红豆杉属植物细胞培养技术的建立和优化[J]. 中国药科大学学报, 1994, (6): 321-324. |
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| 作者姓名： | 高山林 Tow. GHN |
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| 作者单位： | 中国药科大学中药研究所，加拿大哥伦比亚大学植物系 |
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| 摘 要： | 对两种红豆杉属植物细胞培养技术进行了研究，建立了Ｔａｘｕｓｍｅｄｉａ和Ｔａｘｕｓｂｒｅｖｉｆｏｌｉａ的细胞培养技术和方法。通过培养基优化筛选试验成功地诱导获得了１２个细胞株系，通过不同激素试验使细胞生长量大幅度提高，细胞月增长量达１３倍，每升培养基每月干细胞产量达１６．１１ｇ，取得了较好的培养效果，为细胞培养工厂化生产提供了理论依据，为扩大高效抗癌植物资源红豆杉提供了新途径。
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| 关 键 词： | 红豆杉醇 细胞培养 |
Establishment and Optimization of Cell Culture of Taxus Sp. |
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| Establishment and Optimization of Cell Culture of Taxus Sp.[J]. Journal of China Pharmaceutical University, 1994, (6): 321-324. |
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| Authors: | Gao Shanlin Tower GHN |
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| Affiliation: | Gao Shanlin,Tower GHN1 |
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| Abstract: | A study on cell culture of Taxus media and T.brevifolia,which mainly grow in North American with high content of taxol,was done in methods and technique.Twelve different kinds of cell lnies were induced and established from explants Of the species. The growth rate of cell culture increased greately as high as 13 times in 30 d by optimization experiments on suitable media compositions,concentration of phytohormone and culture condition.16.11g dry cell can be produced in one month culture in a litre optimized media.The obtained results provide the possibility of cell culture in industrial scale production and it also paves a new alternative way to produce taxol-the most promising anticancer drug. |
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| Keywords: | Taxol Cell culture Taxus media Taxus brevifolia |
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